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Rabbit Anti-SPP1 Polyclonal Antibody#abs135915

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Description

Catalog-specification	Delivery time	USD price
abs135915-50ug	1-2 Weeks	201.0
abs135915-100ug	1-2 Weeks	301.0

Overview
catalog	abs135915
Description	Osteopontin, also known as bone sialoprotein 1, urinary stone protein, spp-1, Eta-1, nephropontin and uropontin, is a phosphoglycoprotein that plays a vital role in cell adhesion within the extracellular matrix. It is mostly produced by osteoblasts, but brain and kidney cells also synthesize it. OPN is primarily found at various tissue interfaces, including cement lines, lamina limitans, and between collagen fibrils of fully matured hard tissues. Before calcification, OPN is deposited into the unmineralized matrix, which causes its localization. Its molecular weight varies depending on the type of tissue it's secreted from, as it undergoes posttranslational modifications. OPN is a substrate for transglutaminase, and it aids in cell adhesion, chemoattraction, and immunomodulation.
Other names	BNSP, also known as Bone sialoprotein 1 or BSP I, is a protein that plays a role in various biological processes. It is also known by other names such as Early T lymphocyte activation 1 or ETA 1. Another name for this protein is Nephropontin or OPN. It is also referred to as Osteopontin and has been identified as a fusion protein between osteopontin and immunoglobulin alpha 1 heavy chain constant region. This fusion protein is encoded by the gene OSTP_HUMAN or MGC110940. Secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) is another name for this protein. It is often referred to as SPP 1 or SPP-1. Additionally, it is also known as SPP1/CALPHA1 fusion due to its fusion with the CALPHA1 gene. Another name for this protein is Urinary stone protein or Uropontin. By rearranging the provided content, the generated similar content could be: Bone sialoprotein 1 (BSP I) or BNSP, also known as Early T lymphocyte activation 1 (ETA1) or ETA 1, is a protein with various names such as Nephropontin or OPN. It can also be referred to as Osteopontin or as a fusion protein of osteopontin and immunoglobulin alpha 1 heavy chain constant region. This fusion protein is encoded by the gene OSTP_HUMAN or MGC110940. Another name for this protein is Secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1). It is commonly known as SPP 1 or SPP-1. Moreover, it is recognized as SPP1/CALPHA1 fusion since it is fused with the CALPHA1 gene. It is also called Urinary stone protein or Uropontin in some contexts.
Source	Rabbit
Specificity	The detection of endogenous SPP1 levels is possible using the SPP1 Antibody, which specifically targets total SPP1. This antibody enables the accurate measurement and analysis of SPP1 protein expression.
Species Reactivity	Human;Mouse;Rat
Antigen	SPP1
Application	The dilution ranges for the WB test are 1:500 to 1:2000, for the IHC test are 1:50 to 1:200, for the IF/ICC test are 1:100 to 1:500, and for the ELISA (peptide) test are 1:20000 to 1:40000. These dilution ranges are essential for determining the optimal concentration of antibodies to use in each assay. By diluting the antibodies within these ranges, we can achieve accurate and reliable results in detecting and quantifying specific proteins or peptides of interest. It is important to carefully prepare the antibody dilutions to ensure the best sensitivity and specificity in the respective assays.
Immunogen	A synthesized peptide derived from human SPP1.
MW	33kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Tissue specificity	Bone. Found in plasma.
Posttranslational modification	Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif.N- and O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62.
Celluar localization	Endoplasmic reticulum;Extracellular region or secreted;Golgi apparatus;
UniPort	P10451