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Phospho-PKD2 (Ser812) Rabbit Polyclonal Antibody#abs139921

Please be advised that the price provided is for reference only, and for more detailed pricing information, please kindly get in touch with our seller, Vecent. Western blot analysis PKD2 (Phospho-Ser812) using PMA treated Jurkat whole cell lysates This product is for research use only, not for...

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Description

Catalog-specification	Delivery time	USD price
abs139921-100ug	1-2 Weeks	301.0
abs139921-50ug	1-2 Weeks	201.0

Please be advised that the price provided is for reference only, and for more detailed pricing information, please kindly get in touch with our seller, Vecent.

Overview
catalog	abs139921
Other names	The Autosomal dominant polycystic kidney disease type II is also known as APKD2, PKD2, or Polycystic kidney disease 2. The protein involved in this disease is the Polycystic kidney disease 2 protein, also known as PC2 or Polycystin 2. Other names for this protein include PKD4 and Polycystwin. The gene that codes for this protein is called the PKD2 gene and it is located on chromosome 4. Polycystin 2 is a member of the transient receptor potential cation channel subfamily P, also known as TRPP2. Other names for the Polycystin 2 transient receptor potential cation channel include MGC138466 and MGC138468. This disease is inherited in an autosomal dominant manner, meaning that only one copy of the mutated gene is needed to develop the disease. Symptoms of this disease include the formation of numerous cysts in the kidneys, leading to kidney failure.
Source	Rabbit
Specificity	The expression of PKD2 protein can be detected using the PKD2 (Phospho-Ser812) Antibody, specifically when it is phosphorylated at the Ser812 site. This antibody is capable of detecting the endogenous levels of phosphorylated PKD2 protein at Ser812.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Chicken;Rabbit;Xenopus;Dog;Horse;Sheep;Bovine
Antigen	PKD2
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A novel peptide has been developed by utilizing the human PKD2 protein (specifically targeting the phosphorylated site at Ser812). This synthesized peptide is a result of rearranging the original information and serves as a promising advancement in the field.
MW	96KD
Properties
Concentration	1mg/ml
purification	The process of obtaining the antibody involved purifying rabbit serum and using sequential chromatography on both phospho- and non-phospho-peptide affinity columns for affinity purification. The resulting antibody is highly specific and was obtained through a rigorous purification process.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Functions as a cation channel involved in fluid-flow mechanosensation by the primary cilium in renal epithelium (PubMed:18695040). Functions as outward-rectifying K+ channel, but is also permeable to Ca2+, and to a much lesser degree also to Na+ (PubMed:11854751, PubMed:15692563, PubMed:27071085, PubMed:27991905). May contribute to the release of Ca2+ stores from the endoplasmic reticulum (PubMed:11854751, PubMed:20881056). Together with TRPV4, forms mechano- and thermosensitive channels in cilium (PubMed:18695040). PKD1 and PKD2 may function through a common signaling pathway that is necessary to maintain the normal, differentiated state of renal tubule cells. Acts as a regulator of cilium length, together with PKD1. The dynamic control of cilium length is essential in the regulation of mechanotransductive signaling. The cilium length response creates a negative feedback loop whereby fluid shear-mediated deflection of the primary cilium, which decreases intracellular cAMP, leads to cilium shortening and thus decreases flow-induced signaling. Also involved in left-right axis specification via its role in sensing nodal flow; forms a complex with PKD1L1 in cilia to facilitate flow detection in left-right patterning. Detection of asymmetric nodal flow gives rise to a Ca2+ signal that is required for normal, asymmetric expression of genes involved in the specification of body left-right laterality (By similarity).
Tissue specificity	Detected in fetal and adult kidney (PubMed:10770959). Detected at the thick ascending limb of the loop of Henle, at distal tubules, including the distal convoluted tubule and cortical collecting tubules, with weak staining of the collecting duct (PubMed:10770959). Detected on placenta syncytiotrophoblasts (at protein level) (PubMed:26269590). Strongly expressed in ovary, fetal and adult kidney, testis, and small intestine. Not detected in peripheral leukocytes.
Posttranslational modification	Phosphorylated. Phosphorylation is important for protein function; a mutant that lacks the N-terminal phosphorylation sites cannot complement a zebrafish pkd2-deficient mutant (PubMed:16551655). PKD-mediated phosphorylation at the C-terminus regulates its function in the release of Ca2+ stores from the endoplasmic reticulum (PubMed:20881056). PKA-mediated phosphorylation at a C-terminal site strongly increases the open probability of the channel, but does not increase single channel conductance (PubMed:26269590).N-glycosylated. The four subunits in a tetramer probably differ in the extent of glycosylation; simultaneous glycosylation of all experimentally validated sites would probably create steric hindrance. Thus, glycosylation at Asn-305 is not compatible with glycosylation at Asn-328; only one of these two residues is glycosylated at a given time.
Celluar localization	Cytoskeleton;Endoplasmic reticulum;Extracellular region or secreted;Plasma Membrane;
UniPort	Q13563