Rabbit Anti-SIRT3 Polyclonal Antibody#abs131213

abs131213-50ug

1-2 Weeks

201

abs131213-100ug

1-2 Weeks

301

Description

This protein is a NAD-dependent deacetylase that plays a crucial role in activating various mitochondrial target proteins such as ACSS1, IDH2, and GDH by deacetylating specific lysine residues. Its activity significantly influences cellular energy metabolism and is essential for regulating ATP levels within tissues. Thus, this protein is a vital regulator of tissue-specific energy metabolism.

Other names

Sirtuin 3, also known as hSIRT3 or mitochondrial nicotinamide adenine dinucleotide dependent deacetylase, is a regulatory protein that plays a crucial role in mitochondrial function. It belongs to the SIR2 family of proteins, which are homologs of the silent mating type information regulation 2 protein found in Saccharomyces cerevisiae. SIRT3 is a NAD-dependent deacetylase that primarily localizes to the mitochondria.
This protein, also referred to as SIR2L3 or SIR3, is involved in various cellular processes, including metabolism, energy production, and aging. It functions by removing acetyl groups from protein substrates, thereby regulating their activities and impacting cellular physiology. SIRT3 plays a vital role in maintaining mitochondrial integrity and function, as well as modulating cellular responses to stress and nutrient availability.
Sirtuin 3, or SIRT3, is a pivotal player in the intricate network of cellular processes. Its significance lies in its ability to deacetylate target proteins in a NAD-dependent manner, thereby influencing diverse cellular functions. With its resemblance to the yeast SIR2 protein, SIRT3 has garnered attention as a potential intervention target for age-related diseases and metabolic disorders.
In summary, SIRT3 is a mitochondrial NAD-dependent deacetylase that functions as a regulatory protein in various cellular processes. Its importance in maintaining mitochondrial function and cellular homeostasis makes it a fascinating subject of study for researchers exploring interventions against age-related ailments.

Source

Rabbit

Specificity

The total levels of endogenous SIRT3 can be effectively detected using the SIRT3 antibody.

Application

The recommended dilutions for Western blotting range from 1:500 to 1:2000, while for immunohistochemistry, the optimal dilutions are between 1:50 and 1:200. For immunofluorescence/immunocytochemistry, dilutions of 1:100 to 1:500 are suggested. When it comes to ELISA using peptide antigens, the recommended dilutions are in the range of 1:20000 to 1:40000.

A synthesized peptide derived from human SIRT3.

Properties

1mg/ml

Purification

SulfoLink™ Coupling Resin was utilized to perform peptide affinity chromatography for the purification of the antiserum.

Clonality

Polyclonal Antibody

Please make sure to store at -20 °C for a duration of one year to maintain quality. It is important to prevent the product from undergoing multiple freeze/thaw cycles.

Storage buffer

The storage conditions for Rabbit IgG are as follows: it should be kept at a temperature of -20 °C. The IgG should be dissolved in phosphate buffered saline, pH 7.4, with the addition of 150 mM NaCl, 0.02% sodium azide, and 50% glycerol. This solution is stable for a period of 12 months starting from the date of receipt. Please note that it is important to store the IgG under these specified conditions to maintain its integrity and effectiveness.

Target

Background

The NAD-dependent protein deacetylase is a crucial enzyme involved in the activation or deactivation of mitochondrial target proteins. Its role is to remove acetyl groups from key lysine residues, thereby affecting protein function. Several known targets of this deacetylase include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA, and the ATP synthase subunit ATP5O.
By deacetylating these target proteins, this enzyme plays a vital role in regulating cellular energy metabolism. It helps maintain the appropriate balance of ATP, the cellular energy currency, by controlling the activity of specific proteins involved in energy production and utilization. This regulation is particularly important for ensuring tissue-specific levels of ATP, as different tissues have varying energy demands.
In summary, the NAD-dependent protein deacetylase is an essential regulator of cellular energy metabolism. Through deacetylation of key lysine residues in mitochondrial target proteins, it contributes to the control of ATP levels in different tissues, ultimately impacting their specific energy requirements.

Tissue specificity

Widely expressed.

Celluar localization

Mitochondrion;

Q9NTG7