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Rabbit Anti-RPA49 Polyclonal Antibody#abs138977

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Description

Catalog-specification	Delivery time	USD price
abs138977-100ug	1-2 Weeks	301.0
abs138977-50ug	1-2 Weeks	201.0

Overview
catalog	abs138977
Other names	The DNA-directed RNA polymerase I subunit RPA49, also known as POLR1E or Polymerase (RNA) I associated factor 1, is a 53kDa protein involved in the transcription of ribosomal DNA. It is encoded by the gene FLJ13970 and has also been referred to as PAF53 and RNA polymerase I-associated factor 53. In humans, the RNA polymerase I subunit A49 is homologous to this protein. Another name for RPA49 is RNA polymerase I polypeptide E, which is a crucial component of the RNA polymerase I complex. Finally, the protein is also referred to as PRAF1, which stands for RNA polymerase I-associated factor 1.
Source	Rabbit
Specificity	The RPA49 Antibody is designed to recognize and measure the levels of total RPA49 proteins naturally present in a biological sample. This antibody specifically binds to endogenous RPA49 proteins, allowing for the detection and analysis of their expression levels. By targeting RPA49, researchers can gain insights into its role in cellular processes and investigate its potential involvement in various diseases. This antibody provides a valuable tool for studying the function and regulation of RPA49 in biological systems.
Species Reactivity	Human
Antigen	RPA49
Application	ELISA (peptide) 1:20000-1:40000, WB 1:1000-3000, and IF/ICC 1:100-1:500 are the recommended dilution ranges for different immunological techniques. These dilutions are essential for obtaining accurate and reliable results. ELISA (peptide) requires the lowest dilution, ranging from 1:20000 to 1:40000, while WB requires a higher dilution range of 1:1000-3000. IF/ICC falls in the middle with a dilution range of 1:100-1:500. It is important to follow these guidelines to ensure successful detection of the desired protein or antigen.
Immunogen	A synthesized peptide derived from human RPA49.
MW	54 kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized for peptide affinity chromatography to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure long-term preservation, it is best to store at a temperature of -20 °C for up to one year. It is important to avoid exposing the material to repeated cycles of freezing and thawing, as this could potentially damage its integrity. Therefore, it is recommended to take proper measures in handling and storing the material to maintain its quality over time.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF (By similarity).
Celluar localization	Nucleus;
UniPort	Q9GZS1