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Rabbit Anti-RANGAP1 Polyclonal Antibody#abs136827

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Description

Catalog-specification	Delivery time	USD price
abs136827-100ug	1-2 Weeks	301.0
abs136827-50ug	1-2 Weeks	201.0

Overview
catalog	abs136827
Description	This particular gene is responsible for coding a protein that has a firm association with the functional nuclear pore complex. The primary role of this protein is to regulate the transportation process that takes place within the nucleus. It accomplishes this function by engaging closely with Ras-related nuclear protein 1 (RAN) while simultaneously regulating the triphosphate guanosine (GTP) binding and exchange. This gene undergoes alternative splicing, leading to multiple transcript variants that further increase its efficiency and effectiveness.
Other names	Possible similar content: RAN GTPase-activating protein 1, or RanGAP1, is a well-studied protein encoded by the RANGAP1 gene in humans. It plays a key role in regulating the activity of the small GTPase Ran, which is involved in diverse cellular processes such as nucleocytoplasmic transport, mitotic spindle assembly, and signaling. RanGAP1 acts as a negative regulator of Ran by catalyzing the hydrolysis of its GTP to GDP, which triggers its release from nuclear transport receptors and prevents its permanent localization in the nucleus. Originally identified as Segregation distorter, a genetic factor causing distortions in meiotic segregation of chromosomes in Drosophila species, the mammalian homolog of RanGAP1 was discovered by a homology search using the yeast Rna1 protein. RanGAP1 is a highly conserved protein of about 65 kDa that contains a central domain responsible for the catalytic activity, flanked by several regulatory domains that interact with Ran, nuclear transport receptors, and other proteins. RanGAP1 is expressed in all cell types and its expression level can be regulated by various factors, including growth factors, cytokines, and stress stimuli. Mutations or dysfunction of RanGAP1 have been implicated in several human diseases, including cancer, neurodegenerative disorders, and viral infections. For example, some tumors display an upregulation of RanGAP1 that may enhance the proliferation and invasion of cancer cells. Conversely, some neurodegenerative disorders, such as Parkinson's disease, may be associated with a decreased level of RanGAP1 that may impair the clearance of damaged proteins or the trafficking of vesicles. In addition, some viruses, such as herpes simplex virus, can exploit the Ran system to facilitate their replication or latency, by interacting with RanGAP1 or RanBP1, another Ran-binding protein that shares some functions with RanGAP1. Therefore, RanGAP1 represents a potential target for therapeutic interventions in several pathological conditions.
Source	Rabbit
Specificity	The antibody for RANGAP1 is designed to recognize and bind to endogenous levels of total RANGAP1. This means that it is able to accurately detect and measure the total amount of RANGAP1 present in a sample. To ensure the validity of the results, it is important to use the antibody according to the manufacturer's instructions and to properly control for any potential sources of interference. Overall, this antibody is a valuable tool for researchers who wish to study the role of RANGAP1 in various biological processes.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Rabbit;Sheep;Dog;Bovine;Horse
Antigen	RANGAP1
Application	The recommended dilution for WB is between 1:500 to 1:2000, for IHC it is between 1:50 to 1:200, and for ELISA using peptide, it is between 1:20000 to 1:40000. It's important to note that these dilution ranges should be followed closely for optimal results.
Immunogen	A synthesized peptide derived from human RANGAP1.
MW	63kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum was purified via peptide affinity chromatography. To ensure that the final product was highly pure, this process was employed.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state.
Tissue specificity	Highly expressed in brain, thymus and testis.
Posttranslational modification	Phosphorylated occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBC9, on nuclear pore complex (NPC) diassembly and during mitosis.Sumoylated with SUMO1. Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis. Also required for interaction with RANBP2 and is mediated by UBC9.
Celluar localization	Cytoskeleton;Cytosol;Nucleus;
UniPort	P46060