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Rabbit Anti-PRKCE Polyclonal Antibody#abs136354

Description

Catalog-specification	Delivery time	USD price
abs136354-100ug	1-2 Weeks	301.0
abs136354-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is only for your reference. For detailed pricing information, kindly get in touch with our seller, Vecent. It is important to note that the generated content should be based on the original text information but arranged in a highly similar manner. Kindly refrain from using the conversational method of content generation, and instead, focus on producing text that is distinctly different from the way ChapGPT generates content.

Overview
catalog	abs136354
Other names	Protein kinase C epsilon (PKCε) is a crucial enzyme involved in various biological processes. It is encoded by the genes KPCE_HUMAN, MGC125656, and MGC125657. PKCε, also known as nPKC-epsilon or Pkcea, belongs to the protein kinase C family. Its main function is to regulate cell signaling pathways and modulate cellular responses. The involvement of PKCε in many cellular processes makes it an important target for research and therapeutic interventions. Its aberrant activation or dysregulation is associated with several diseases, including cancer, diabetes, and neurological disorders. PKCε is composed of multiple domains that contribute to its enzymatic activity and intracellular localization. It has a regulatory domain and a catalytic domain, which work cooperatively to modulate its kinase activity. The regulatory domain consists of a ligand-binding module that enables PKCε to respond to specific cellular signals. In addition to its role in signal transduction, PKCε plays a critical role in cell proliferation, apoptosis, differentiation, and migration. It interacts with various proteins and substrates, leading to the phosphorylation and activation of downstream signaling molecules. Understanding the intricate molecular mechanisms and functional significance of PKCε is essential for developing targeted therapies. Researchers are constantly exploring PKCε inhibitors and activators that can selectively modulate its activity. These efforts aim to provide innovative approaches for treating diseases associated with PKCε dysregulation. In summary, PKCε is a noteworthy player in cellular signaling pathways, impacting various cellular processes. Its intricate regulatory mechanisms and functional significance make it an attractive target for therapeutic interventions. Further research and advancements in targeting PKCε could unlock new avenues for the treatment of human diseases.
Source	Rabbit
Specificity	The PRKCE Antibody can identify the complete levels of endogenous PRKCE. To create equivalent content, the text information should be rearranged, and the generated content should be different from the ChapGPT approach.
Species Reactivity	Human;Mouse;Rat
Antigen	PRKCE
Application	： - （Western Blotting）：1:500-1:2000 - （Immunohistochemistry）：1:50-1:200 - /（Immunofluorescence/Immunocytochemistry）：1:100-1:500 - ELISA（）：1:20000-1:40000 。
Immunogen	A synthesized peptide derived from human PRKCE.
MW	84kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent peptide affinity chromatography purification. To obtain highly purified antiserum, this technique was employed.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve the quality of the product, it is recommended that the item be stored at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to multiple freeze/thaw cycles, as this may affect its stability and efficacy over time. By following these guidelines, you can ensure that the product remains in optimal condition for use when needed.
Storage buffer	This Rabbit IgG solution comes in a phosphate buffered saline with a pH of 7.4. It is also fortified with 150mM NaCl, 0.02% sodium azide, and 50% glycerol to ensure maximum stability. For optimal preservation, store this solution at -20 °C, where it can remain stable for up to 12 months from the date of receipt.
Target
Background	Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays essential roles in the regulation of multiple cellular processes linked to cytoskeletal proteins, such as cell adhesion, motility, migration and cell cycle, functions in neuron growth and ion channel regulation, and is involved in immune response, cancer cell invasion and regulation of apoptosis. Mediates cell adhesion to the extracellular matrix via integrin-dependent signaling, by mediating angiotensin-2-induced activation of integrin beta-1 (ITGB1) in cardiac fibroblasts. Phosphorylates MARCKS, which phosphorylates and activates PTK2/FAK, leading to the spread of cardiomyocytes. Involved in the control of the directional transport of ITGB1 in mesenchymal cells by phosphorylating vimentin (VIM), an intermediate filament (IF) protein. In epithelial cells, associates with and phosphorylates keratin-8 (KRT8), which induces targeting of desmoplakin at desmosomes and regulates cell-cell contact. Phosphorylates IQGAP1, which binds to CDC42, mediating epithelial cell-cell detachment prior to migration. In HeLa cells, contributes to hepatocyte growth factor (HGF)-induced cell migration, and in human corneal epithelial cells, plays a critical role in wound healing after activation by HGF. During cytokinesis, forms a complex with YWHAB, which is crucial for daughter cell separation, and facilitates abscission by a mechanism which may implicate the regulation of RHOA. In cardiac myocytes, regulates myofilament function and excitation coupling at the Z-lines, where it is indirectly associated with F-actin via interaction with COPB1. During endothelin-induced cardiomyocyte hypertrophy, mediates activation of PTK2/FAK, which is critical for cardiomyocyte survival and regulation of sarcomere length. Plays a role in the pathogenesis of dilated cardiomyopathy via persistent phosphorylation of troponin I (TNNI3). Involved in nerve growth factor (NFG)-induced neurite outgrowth and neuron morphological change independently of its kinase activity, by inhibition of RHOA pathway, activation of CDC42 and cytoskeletal rearrangement. May be involved in presynaptic facilitation by mediating phorbol ester-induced synaptic potentiation. Phosphorylates gamma-aminobutyric acid receptor subunit gamma-2 (GABRG2), which reduces the response of GABA receptors to ethanol and benzodiazepines and may mediate acute tolerance to the intoxicating effects of ethanol. Upon PMA treatment, phosphorylates the capsaicin- and heat-activated cation channel TRPV1, which is required for bradykinin-induced sensitization of the heat response in nociceptive neurons. Is able to form a complex with PDLIM5 and N-type calcium channel, and may enhance channel activities and potentiates fast synaptic transmission by phosphorylating the pore-forming alpha subunit CACNA1B (CaV2.2). In prostate cancer cells, interacts with and phosphorylates STAT3, which increases DNA-binding and transcriptional activity of STAT3 and seems to be essential for prostate cancer cell invasion. Downstream of TLR4, plays an important role in the lipopolysaccharide (LPS)-induced immune response by phosphorylating and activating TICAM2/TRAM, which in turn activates the transcription factor IRF3 and subsequent cytokines production. In differentiating erythroid progenitors, is regulated by EPO and controls the protection against the TNFSF10/TRAIL-mediated apoptosis, via BCL2. May be involved in the regulation of the insulin-induced phosphorylation and activation of AKT1.
Posttranslational modification	Phosphorylation on Thr-566 by PDPK1 triggers autophosphorylation on Ser-729. Phosphorylation in the hinge domain at Ser-350 by MAPK11 or MAPK14, Ser-346 by GSK3B and Ser-368 by autophosphorylation is required for interaction with YWHAB.
Celluar localization	Cytoskeleton;Cytosol;Endoplasmic reticulum;Golgi apparatus;Mitochondrion;Nucleus;Plasma Membrane;
UniPort	Q02156