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Rabbit Anti-POU4F1 Polyclonal Antibody#abs135073

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Description

Catalog-specification	Delivery time	USD price
abs135073-100ug	1-2 Weeks	301.0
abs135073-50ug	1-2 Weeks	201.0

Overview
catalog	abs135073
Other names	Brain-specific homeobox/POU domain protein 3A, also known as Brain-3A or Brn-3A, is a transcription factor that belongs to the POU class 4 homeobox family. It is encoded by the gene POU4F1. This protein plays a crucial role in the development and function of the brain. It is involved in regulating the expression of various target genes that are important for neuronal growth, survival, and differentiation. Brn-3A is highly expressed in specific regions of the brain, such as the retina and cochlea. It acts as a key regulator of sensory neuron development and maintenance in these regions. Additionally, it has been found to be essential for the proper formation of auditory and visual pathways during embryonic development. Studies have shown that mutations or dysregulation of Brn-3A can lead to various neurological disorders, including hearing loss and visual impairment. It is also implicated in neurodegenerative diseases, such as Parkinson's and Alzheimer's disease. Further research is being conducted to explore the precise molecular mechanisms by which Brn-3A functions, as well as its potential as a therapeutic target for neurological conditions.
Source	Rabbit
Specificity	The POU4F1 Antibody is specifically designed to detect total POU4F1 protein levels that naturally occur within an organism. It is capable of identifying endogenous POU4F1 expression accurately. By utilizing this antibody, researchers can gain insights into the overall levels of POU4F1 protein present in a biological sample. These measurements can be crucial in understanding the role and function of POU4F1 in various cellular processes.
Species Reactivity	Human;Mouse;Rat
Antigen	POU4F1
Application	WB 1:500~1:1000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide.
MW	42 KD
Properties
Concentration	1mg/ml
purification	Peptide affinity chromatography using SulfoLink™ Coupling Resin was employed for the purification of the antiserum. Let's generate another content using a different approach while ensuring that it is based on the original information: To obtain a purified antiserum, we utilized SulfoLink™ Coupling Resin in peptide affinity chromatography. This technique allowed us to remove impurities and enhance the quality of the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure optimum preservation, it is recommended to store the item at a temperature of -20 °C for a period of one year. It is important to avoid exposing the item to repeated cycles of freezing and thawing, as this could affect its quality and efficacy. Therefore, it is essential to handle the item with care and follow the recommended guidelines for storage and handling.
Storage buffer	The storage instructions for Rabbit IgG are as follows: it should be stored at -20 °C in phosphate buffered saline with a pH of 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. The stability of the IgG is guaranteed for 12 months from the date of receipt.
Target
Background	Multifunctional transcription factor with different regions mediating its different effects. Acts by binding (via its C-terminal domain) to sequences related to the consensus octamer motif 5'-ATGCAAAT-3' in the regulatory regions of its target genes. Regulates the expression of specific genes involved in differentiation and survival within a subset of neuronal lineages. It has been shown that activation of some of these genes requires its N-terminal domain, maybe through a neuronal-specific cofactor. Ativates BCL2 expression and protects neuronal cells from apoptosis (via the N-terminal domain). Induces neuronal process outgrowth and the coordinate expression of genes encoding synaptic proteins. Exerts its major developmental effects in somatosensory neurons and in brainstem nuclei involved in motor control. Stimulates the binding affinity of the nuclear estrogene receptor ESR1 to DNA estrogen response element (ERE), and hence modulates ESR1-induced transcriptional activity. May positively regulate POU4F2 and POU4F3. Regulates dorsal root ganglion sensory neuron specification and axonal projection into the spinal cord. Plays a role in TNFSF11-mediated terminal osteoclast differentiation. Negatively regulates its own expression interacting directly with a highly conserved autoregulatory domain surrounding the transcription initiation site.
Tissue specificity	Expressed in the brain and the retina. Present in the developing brain, spinal cord and eye.
Celluar localization	Nucleus;
UniPort	Q01851