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Rabbit Anti-POLR2I Polyclonal Antibody#abs136188

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Description

Catalog-specification	Delivery time	USD price
abs136188-100ug	1-2 Weeks	301.0
abs136188-50ug	1-2 Weeks	201.0

Overview
catalog	abs136188
Description	RNA polymerase II is a crucial enzyme in eukaryotes responsible for synthesizing messenger RNA. One of its essential components is encoded by a specific gene. Together with two other subunits, this gene's product forms the DNA binding domain of the polymerase. This domain contains a groove wherein the DNA template is transcribed into RNA. Notably, the subunit of interest possesses zinc binding activity and features two zinc finger motifs with conserved cysteines. These details highlight the significance and functional characteristics of this specific gene. The provided information is from RefSeq and dates back to July 2008.
Other names	The 14.5 kDa polypeptide of DNA directed RNA polymerase II, also known as POLR2I or RPB9, plays a crucial role in transcriptional processes. It is an essential subunit of the DNA directed RNA polymerase II enzyme, responsible for synthesizing RNA transcripts from DNA templates. This subunit, designated as RPB9 or hRPB14.5, is involved in various regulatory mechanisms that control gene expression. The DNA directed RNA polymerase II polypeptide I, also referred to as DNA directed RNA polymerase II subunit I, is another component of the RNA polymerase II enzyme. This subunit, along with RPB9, assists in the assembly and stability of the polymerase complex. Together, they contribute to the accurate and efficient transcription of DNA into RNA. The significance of these subunits in the functioning of RNA polymerase II cannot be overstated. They provide structural integrity and contribute to the catalytic activity of the enzyme. Through interactions with other subunits and transcription factors, they facilitate the recognition of promoter sequences, initiation of transcription, and processivity of RNA synthesis. Understanding the roles and interactions of these subunits is crucial for unraveling the complexities of gene regulation and the mechanisms underlying diseases associated with transcriptional dysregulation. Further research in this area promises to shed light on the intricate processes governing gene expression and open doors to potential therapeutic interventions.
Source	Rabbit
Specificity	The POLR2I Antibody is capable of detecting the total POLR2I levels that naturally occur within an organism. To emphasize, it identifies the endogenous amounts of POLR2I protein. To reiterate, our antibody specifically targets and recognizes the endogenous levels of total POLR2I protein.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Zebrafish;Chicken;Rabbit;Pig;Dog;Horse;Bovine;Sheep;
Antigen	POLR2I
Application	For Western blot analysis, dilute the primary antibody at a range of 1:500-1:2000. When working with Immunohistochemistry, the recommended dilution of primary antibody is within the range of 1:50-1:200. For ELISA assays using a peptide antigen, dilutions of the primary antibody should be made between 1:20000-1:40000. It is important to follow these guidelines to ensure accurate and reliable results in your experiments. Adjusting the dilution appropriately can prevent issues such as non-specific binding or weak signal intensity. By optimizing your primary antibody concentration, you can improve the sensitivity and specificity of your assays.
Immunogen	A synthesized peptide derived from human POLR2I.
MW	15kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB9 is part of the upper jaw surrounding the central large cleft and thought to grab the incoming DNA template (By similarity).
Celluar localization	Nucleus;
UniPort	P36954