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Rabbit Anti-PNN Polyclonal Antibody#abs134598

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Description

Catalog-specification	Delivery time	USD price
abs134598-100ug	1-2 Weeks	301.0
abs134598-50ug	1-2 Weeks	201.0

Overview
catalog	abs134598
Other names	Pinin, also known as nuclear protein SDK3 or SR-like protein, is a 140 kDa phosphoprotein that plays important roles in nuclear and cell adhesion processes. It serves as a desmosome-associated protein and is involved in maintaining the integrity of cell-to-cell junctions. Pinin is also known as domain-rich serine protein due to its high content of serine residues. It is encoded by the DRS gene and is sometimes referred to as DRS protein or DRSP. Another name for Pinin is melanoma metastasis clone A protein (MEMA), highlighting its relevance in melanoma progression. Its presence in the nucleus indicates its nuclear functions and involvement in gene regulation. Pinin is commonly referred to by its abbreviation PNN. With its diverse array of roles, Pinin has garnered significant interest in the research community. Its precise functions and contributions to various cellular processes are still being elucidated, making it an intriguing candidate for further investigation.
Source	Rabbit
Specificity	The endogenous levels of total PNN can be accurately measured using the PNN Antibody.
Species Reactivity	Human;Mouse
Predictive reaction species	Xenopus;Rabbit;Pig;Dog;Sheep;Bovine;Horse
Antigen	PNN
Application	For western blotting, a dilution range of 1:500 to 1:1000 is recommended, while for immunofluorescence or immunocytochemistry, a dilution range of 1:100 to 1:500 is suggested. When performing ELISA using peptide as the antigen, a dilution range of 1:20000 to 1:40000 is recommended for best results. It is important to note that proper dilutions should be determined through experimentation and may vary depending on the specific experimental setup.
Immunogen	A synthesized peptide.
MW	85 KD
Properties
Concentration	1mg/ml
purification	The purification of the antiserum involved utilizing SulfoLink™ Coupling Resin for peptide affinity chromatography. This process effectively removed impurities and ensured a purified end product.
Clonality	Polyclonal Antibody
Stability & Storage	One year of storage is recommended at -20 °C to maintain the quality of the product. It is important to avoid subjecting the product to repeated freeze and thaw cycles.
Storage buffer	The storage conditions for Rabbit IgG are as follows: it is suspended in phosphate buffered saline with a pH of 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at a temperature of -20 °C. Its stability is guaranteed for a period of 12 months from the date of receipt.
Target
Background	Transcriptional activator binding to the E-box 1 core sequence of the E-cadherin promoter gene; the core-binding sequence is 5'CAGGTG-3'. Capable of reversing CTBP1-mediated transcription repression. Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Participates in the regulation of alternative pre-mRNA splicing. Associates to spliced mRNA within 60 nt upstream of the 5'-splice sites. Component of the PSAP complex which binds RNA in a sequence-independent manner and is proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets. Involved in the establishment and maintenance of epithelia cell-cell adhesion. Potential tumor suppressor for renal cell carcinoma.
Tissue specificity	Expressed in placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon, heart, epidermis, esophagus, brain and smooth and skeletal muscle. Expressed strongly in melanoma metastasis lesions and advanced primary tumors.
Celluar localization	Cytoskeleton;Nucleus;Plasma Membrane;
UniPort	Q9H307