Rabbit Anti-PI3-kinase P85-alpha/gamma Polyclonal Antibody#abs131774
Please be advised that the price mentioned is only for your reference. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent. Western blot analysis of PI3-kinase p85-alpha/gamma expression in H2O2 treated COS7 whole cell lysates,The lane on the left is...
Description
| Catalog-specification | Delivery time | USD price |
abs131774-100ug | 1-2 Weeks | 301.0 |
abs131774-50ug | 1-2 Weeks | 201.0 |
Please be advised that the price mentioned is only for your reference. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent.
| Overview | |
catalog | abs131774 |
Description | PIK3R1 functions as a regulatory subunit of phosphoinositide-3-kinase and is responsible for mediating binding to specific tyrosine-phosphorylated proteins via its SH2 domain. It plays a critical adapter role in the association of alpha, beta, and delta enzymes' p110 catalytic units to the plasma membrane, facilitating the phosphorylation of inositol lipids by p110. Additionally, it may play a role in regulating the actin cytoskeleton. |
| Other names | GRB1, also known as p85 alpha, is a regulatory subunit of phosphatidylinositol 3 kinase (PI3K). It is involved in cellular signaling pathways and plays a crucial role in regulating cell growth, proliferation, and survival. The full name of this protein is Phosphatidylinositol 3 kinase associated p85 alpha, and it is encoded by the gene PIK3R1. On the other hand, there is another regulatory subunit, known as p55 gamma or p85 gamma, which is encoded by the gene PIK3R3. This subunit, also a part of PI3K, is called Phosphatidylinositol 3 kinase regulatory subunit gamma. It is involved in similar cellular signaling pathways as p85 alpha. Both p85 alpha and p85 gamma regulate the activity of the catalytic subunit of PI3K, enabling the conversion of phosphatidylinositol 4,5-bisphosphate (PIP2) to phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 acts as a secondary messenger and activates downstream signaling cascades that regulate various cellular processes such as cell growth, differentiation, and survival. In summary, GRB1 or p85 alpha, and p55 gamma or p85 gamma are regulatory subunits of PI3K. They play key roles in modulating cellular signaling pathways and are essential for maintaining proper cell functions. |
| Source | Rabbit |
| Specificity | PI3-kinase p85-alpha/gamma Antibody detects endogenous levels of total PI3-kinase p85-alpha/gamma. |
| Species Reactivity | Human;Mouse;Rat;Monkey |
| Predictive reaction species | Zebrafish;Rabbit; |
| Antigen | PI3-kinase p85-alpha/gamma |
| Application | WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000 |
| Immunogen | A synthesized peptide derived from human PI3-kinase p85-alpha/gamma. |
| MW | 54,83kDa |
| Properties | |
Concentration | 1mg/ml |
| purification | The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin . |
| Clonality | Polyclonal Antibody |
| Stability & Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles |
| Storage buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |
Target | |
Background | Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed:17626883, PubMed:19805105, PubMed:7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348923). |
| Tissue specificity | Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level). |
| Posttranslational modification | Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.Phosphorylated. Tyrosine phosphorylated in response to signaling by FGFR1, FGFR2, FGFR3 and FGFR4. Phosphorylated by CSF1R. Phosphorylated by ERBB4. Phosphorylated on tyrosine residues by TEK/TIE2. Dephosphorylated by PTPRJ. Phosphorylated by PIK3CA at Ser-608; phosphorylation is stimulated by insulin and PDGF. The relevance of phosphorylation by PIK3CA is however unclear (By similarity). Phosphorylated in response to KIT and KITLG/SCF. Phosphorylated by FGR. |
| Celluar localization | Cytosol;Endoplasmic reticulum;Nucleus;Plasma Membrane; |
| UniPort | P27986/Q92569 |
Western blot analysis of PI3-kinase p85-alpha/gamma expression in H2O2 treated COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
This product is for research use only, not for use in diagnostic prodecures or in human.
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