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Rabbit Anti-PGC1 Alpha Polyclonal Antibody#abs131496

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Description

Catalog-specification	Delivery time	USD price
abs131496-100ug	1-2 Weeks	301.0
abs131496-50ug	1-2 Weeks	201.0

Overview
catalog	abs131496
Description	A potent enhancer of gene transcription, the transcriptional coactivator plays a crucial role in amplifying the activity of steroid receptors and nuclear receptors. It exerts a profound impact on the uncoupling protein promoter by significantly increasing the transcriptional activity of PPARG and thyroid hormone receptor. Additionally, this coactivator has the capability to regulate essential mitochondrial genes that contribute to the finely orchestrated program of adaptive thermogenesis.
Other names	The protein known as PGC-1alpha, also referred to as Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1 Alpha, is a key regulator of gene expression involved in various metabolic processes. PGC-1alpha has also been identified as a Ligand Effect Modulator 6 and is vital in the maintenance of homeostasis in various tissues and organs. Alternate names for PGC-1alpha include Peroxisome Proliferative Activated Receptor Gamma Coactivator 1 and PPAR Gamma Coactivator 1 Alpha. The protein exists in multiple transcript variants, including B4 3ext, B4 8a, B4, B5, and B5 NT. PGC-1alpha is essential for the regulation of energy homeostasis in the body, particularly in organs such as the skeletal muscle, liver, and adipose tissue. In addition, it plays a critical role in mitochondrial biogenesis and influences the expression of many genes involved in the oxidative metabolism and fatty acid utilization in the body. Given its significant influence in metabolic processes, PGC-1alpha has been studied extensively as a therapeutic target for treating obesity, diabetes, and other related metabolic disorders.
Source	Rabbit
Specificity	The detection of total PGC1 alpha is possible with the PGC1 alpha antibody, which accurately identifies the endogenous levels of this protein. To generate content similar to the original text but with rearranged information, we can say: The total PGC1 alpha present in a biological sample can be effectively detected using the PGC1 alpha antibody. This antibody specifically recognizes the endogenous levels of PGC1 alpha protein and enables its quantification.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Chicken;Rabbit;Pig;Dog;Bovine;Horse;
Antigen	PGC1 alpha
Application	The recommended dilution for WB is between 1:500-1:2000, with IHC requiring a dilution of 1:50-1:200. In addition, for ELISA using a peptide, it is suggested to use a dilution ranging from 1:20000 to 1:40000. These dilutions will ensure optimal results for the respective applications.
Immunogen	A synthesized peptide derived from human PGC1 alpha.
MW	91 kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK.
Tissue specificity	Heart, skeletal muscle, liver and kidney. Expressed at lower levels in brain and pancreas and at very low levels in the intestine and white adipose tissue. In skeletal muscle, levels were lower in obese than in lean subjects and fasting induced a 2-fold increase in levels in the skeletal muscle in obese subjects.
Posttranslational modification	Phosphorylation by AMPK in skeletal muscle increases activation of its own promoter. Phosphorylated by CLK2.Heavily acetylated by GCN5 and biologically inactive under conditions of high nutrients. Deacetylated by SIRT1 in low nutrients/high NAD conditions.Ubiquitinated. Ubiquitination by RNF34 induces proteasomal degradation.
Celluar localization	Cytosol;Mitochondrion;Nucleus;
UniPort	Q9UBK2