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Rabbit Anti-PABPC5 Polyclonal Antibody#abs134605

Please note that the price provided is only for your reference. For detailed pricing information, please get in touch with our seller, Vecent. We highly recommend contacting Vecent for accurate pricing details. Western blot analysis of extracts from K562 cells, using PABPC5 antibody. This...

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Description

Catalog-specification	Delivery time	USD price
abs134605-100ug	1-2 Weeks	301.0
abs134605-50ug	1-2 Weeks	201.0

Overview
catalog	abs134605
Other names	PABP5, also known as Poly(A) binding protein 5 or Polyadenylate binding protein 5, is a cytoplasmic protein that plays a crucial role in RNA metabolism. This protein specifically binds to the poly(A) tail of mRNA molecules, which helps regulate their stability and translation efficiency. Through its interaction with other factors, PABP5 can influence mRNA localization, degradation, and protein synthesis. It is involved in various cellular processes, including cell growth, development, and stress responses. PABP5's diverse functions highlight its importance in maintaining proper gene expression and contributing to cellular homeostasis. Its dysregulation has been associated with various diseases, making PABP5 an attractive target for therapeutic interventions. Further research is needed to fully elucidate the molecular mechanisms underlying PABP5's functions and exploit its potential for clinical applications.
Source	Rabbit
Specificity	The antibody against PABPC5 recognizes the complete protein present naturally in the cells. There is no alteration in the sequence of the PABPC5 protein being detected by this antibody.
Species Reactivity	Human
Antigen	PABPC5
Application	Here are some possible variations of the original content: - If you're planning to use Western blotting for your experiment, dilute your primary antibody at 1:500 to 1:1000. For immunofluorescence or immunocytochemistry, use a dilution of 1:100 to 1:500. For ELISA using a peptide antigen, the recommended antibody dilution is between 1:20000 and 1:40000. - Dilute your primary antibody accordingly if you're performing Western blotting (1:500 to 1:1000), immunofluorescence or immunocytochemistry (1:100 to 1:500), or ELISA with a peptide antigen (1:20000 to 1:40000). These dilutions can improve the sensitivity and specificity of your assay and reduce background noise. - Depending on the type of immunoassay you're using, the recommended dilutions for your primary antibody may vary. For Western blotting, a dilution of 1:500 to 1:1000 is suitable, while for immunofluorescence or immunocytochemistry, a dilution of 1:100 to 1:500 is suggested. If you're performing ELISA with a peptide antigen, consider using a dilution range of 1:20000 to 1:40000. These guidelines can help you obtain optimal results for your experiments. - Achieving optimal antibody dilution can greatly affect the outcome of your immunoassay, whether it's Western blotting, immunofluorescence, or ELISA with a peptide antigen. Generally, a dilution of 1:500 to 1:1000 is recommended for Western blotting, while a dilution of 1:100 to 1:500 is advised for immunofluorescence or immunocytochemistry. For ELISA with a peptide antigen, a dilution range of 1:20000 to 1:40000 is often used. Make sure to test several dilutions to find the best one for your specific assay.
Immunogen	A synthesized peptide.
MW	43 KD
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent a purification process through peptide affinity chromatography. Through this method, the antiserum was purified to remove any impurities and produce a highly concentrated and effective product.
Clonality	Polyclonal Antibody
Stability & Storage	Please ensure that the product is stored at -20 °C for one year without any exposure to repeated freeze/thaw cycles. It is essential to avoid subjecting the product to fluctuating temperature conditions. Proper storage conditions will help maintain the product's quality and efficacy over time.
Storage buffer	Phosphate buffered saline with rabbit IgG is formulated to have a pH of 7.4 and contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store this solution at -20 °C. The stability of the product is guaranteed for a period of 12 months starting from the date of its receipt.
Target
Background	This protein is responsible for binding to the poly(A) tail of mRNA. It is believed to play a role in regulating mRNA metabolism in the cytoplasm. Additionally, it is possible that it can bind to other RNA sequences within the cytoplasm besides poly(A) in vivo.
Tissue specificity	Expressed in fetal brain and in a range of adult tissues.
Celluar localization	Mitochondrion;
UniPort	Q96DU9