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Rabbit Anti-p44 MAPK Polyclonal Antibody#abs133436

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Description

Catalog-specification	Delivery time	USD price
abs133436-100ug	1-2 Weeks	301.0
abs133436-50ug	1-2 Weeks	201.0

Overview
catalog	abs133436
Other names	ERK1, also known as Extracellular Signal Regulated Kinase 1 or MAPK3, is an important protein kinase involved in various cellular processes. It plays a crucial role in signal transduction pathways and regulates gene expression, cell proliferation, differentiation, and cell survival. ERK1 is a member of the MAP kinase family and is activated by phosphorylation in response to extracellular signals such as growth factors and stress stimuli. The ERK1 protein is encoded by the HGNC6877 gene and is also known by other names including ERK, ERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, Insulin-stimulated MAP2 kinase, MAP kinase 1, MAP kinase 3, MAPK, MAPK1, Mapk3, MGC20180, Microtubule-Associated Protein 2 Kinase, Mitogen-activated protein kinase 1, p44 ERK1, p44 MAPK, p44-ERK1, p44-MAPK, P44ERK1, P44MAPK, PRKM3, and Protein Kinase Mitogen Activated 3. Overall, ERK1 is a versatile kinase that plays a critical role in many cellular processes, making it an important target for therapeutic interventions and further research.
Source	Rabbit
Specificity	The p44 MAPK Antibody is capable of detecting total p44 MAPK at its endogenous levels within the body. Essentially, this antibody is designed to recognize and bind to specific proteins associated with p44 MAPK, providing valuable insight into the processes and mechanisms underlying this important pathway. Through careful analysis and interpretation of the data obtained from p44 MAPK detection using this antibody, researchers can gain a better understanding of the molecular events driving cellular processes and disease.
Species Reactivity	Human;Mouse;Rat
Antigen	p44 MAPK
Application	There is a wide range of dilution ratios used in various biological experiments. For Western Blot (WB), the typical dilution range is between 1:500 to 1:1000. In Immunofluorescence (IF) or Immunocytochemistry (ICC), the dilution ratio commonly falls within the range of 1:100 to 1:500. When it comes to ELISA involving peptides, the recommended dilution range is broader, ranging from 1:20000 to 1:40000. These dilution ratios play a crucial role in achieving accurate results and ensuring the effectiveness of the experimental procedures.
Immunogen	A synthesized peptide.
MW	45 KD
Properties
Concentration	1mg/ml
purification	We utilized SulfoLink™ Coupling Resin in the peptide affinity chromatography process to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.
Posttranslational modification	Phosphorylated upon KIT and FLT3 signaling (By similarity). Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-204.
Celluar localization	Cytoskeleton;Cytosol;Endosome;Extracellular region or secreted;Golgi apparatus;Mitochondrion;Nucleus;Plasma Membrane;
UniPort	P27361