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Rabbit Anti-NTRK1 Polyclonal Antibody#abs136342

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Description

Catalog-specification	Delivery time	USD price
abs136342-100ug	1-2 Weeks	301.0
abs136342-50ug	1-2 Weeks	201.0

Overview
catalog	abs136342
Description	The Trk receptor tyrosine kinases family comprises TrkA, TrkB, and TrkC. Although sharing a highly conserved sequence, these receptors are activated by distinct neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3, respectively. Neurotrophin signaling via these receptors plays a crucial role in mediating several physiological processes such as cell survival, neural development, proliferation, dendrite growth, and axon patterning. In the adult nervous system, the Trk receptors are involved in the regulation of synaptic strength and plasticity. TrkA, in particular, is essential for the development and maturation of the nervous system as well as for regulating cell proliferation. It has been found that phosphorylation at Tyr490 is crucial for Shc association, which leads to the activation of the Ras-MAP kinase cascade. Additionally, Tyrosine residues at positions 674/675 within the catalytic domain reflect TrkA kinase activity. Aberrant TrkA activation caused by point mutations, deletions, and chromosomal rearrangements, may lead to ligand-independent receptor dimerization. Moreover, TrkA is frequently activated in different malignancies including ovarian, breast, prostate, and thyroid carcinomas. Interestingly, TrkA expression appears to be a favorable prognostic marker for neuroblastomas. This is because TrkA signaling leads to growth arrest and differentiation of cells derived from the neural crest, which neuroblastomas originate from.
Other names	The High affinity nerve growth factor receptor, also known as Trk A or NTRK1, is a neurotrophic tyrosine kinase receptor type 1. It is encoded by the NTRK1 gene and known to be an oncogene TRK. The receptor plays an essential role in the development and maintenance of the nervous system, especially slow nerve growth. The receptor has a precursor, p140-TrkA, which is also referred to as p14-TrkA and gp140trk. It belongs to the tropomyosin-related kinase A family and functions as a tyrosine kinase receptor. Overall, Trk A is a crucial protein in promoting nerve growth and maintaining a healthy nervous system.
Source	Rabbit
Specificity	The antibody for NTRK1 is capable of identifying the natural levels of total NTRK1 within an organism. To generate similar content based on the original information, one could rephrase the statement as follows: The NTRK1 Antibody can detect the native expression levels of total NTRK1 in a biological system.
Species Reactivity	Human;Mouse;Rat
Antigen	NTRK1
Application	WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthetic peptideof human NTRK1.
MW	145kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand (PubMed:1850821, PubMed:1849459, PubMed:1281417, PubMed:8325889, PubMed:15488758, PubMed:17196528). Can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival (By similarity). Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation (PubMed:1281417). Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors.
Tissue specificity	Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors.
Posttranslational modification	Ligand-mediated autophosphorylation (PubMed:2927393, PubMed:1281417, PubMed:15488758, PubMed:7510697, PubMed:8155326, PubMed:8325889, PubMed:27676246). Interaction with SQSTM1 is phosphotyrosine-dependent. Autophosphorylation at Tyr-496 mediates interaction and phosphorylation of SHC1 (PubMed:15488758, PubMed:7510697, PubMed:8155326, PubMed:8325889).N-glycosylated (PubMed:2927393). Isoform TrkA-I and isoform TrkA-II are N-glycosylated.Ubiquitinated. Undergoes polyubiquitination upon activation; regulated by NGFR. Ubiquitination regulates the internalization of the receptor.
Celluar localization	Endosome;Plasma Membrane;
UniPort	P04629