enLanguage
Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details
Rabbit Anti-Notch1 (Cleaved-Val1744) Polyclonal Antibody#abs131403

Rabbit Anti-Notch1 (Cleaved-Val1744) Polyclonal Antibody#abs131403

Please note that the price provided is for your reference only. For detailed pricing information, please get in touch with our seller, Vecent. I urge you to reach out to Vecent for precise pricing. Western blot analysis of Notch 1 (Cleaved-Val1744) expression in NIH 3T3 cell extract treated with...

Send Inquiry

Description

Catalog-specificationDelivery timeUSD price

abs131403-100ug

1-2 Weeks

301.0

abs131403-50ug

1-2 Weeks

201.0

Please note that the price provided is for your reference only. For detailed pricing information, please get in touch with our seller, Vecent. I urge you to reach out to Vecent for precise pricing.


Overview

catalog

abs131403

Description

The role of this receptor is to bind membrane-bound ligands Jagged1, Jagged2, and Delta1, thereby playing a crucial role in regulating cell-fate determination. Once the ligands are activated, the released Notch intracellular domain (NICD) interacts with it to form a transcriptional activator complex along with RBPJ/RBPSUH. This complex then activates genes located in the enhancer of split locus. To summarize, this receptor functions as a mediator of signaling pathways, playing a key role in determining cell fate by activating specific genes.

Other namesNotch 1, also known as Neurogenic locus notch homolog protein 1, is a transmembrane protein involved in cell signaling. It is encoded by the gene 9930111A19Rik, AOS5, AOVD1, hN1, Lin-12, LIN12, Mis6, Motch A, mT14, TAN1, xotch and other names. Notch 1 plays a role in regulating cell fate decisions during embryonic development and in adult tissue homeostasis. Its intracellular domain, NICD, is released upon activation and interacts with downstream effectors to drive transcriptional changes. Mutations in Notch 1 have been associated with various human diseases. Despite its complex nature, the structural and functional features of Notch 1 are well studied and continue to be a subject of active research.
SourceRabbit
SpecificityThe antibody called Notch 1 (Cleaved-Val1744) is capable of detecting the total levels of endogenous Notch 1 (Cleaved-Val1744). This detection occurs at the protein level, providing valuable information about the presence of Notch 1 (Cleaved-Val1744) in a sample.
Species ReactivityHuman;Mouse;Rat
Predictive reaction speciesPig;Bovine;Dog;Horse;
AntigenNotch1
ApplicationThe dilution range for Western Blotting (WB) is typically 1:500 to 1:2000. For Immunohistochemistry (IHC), the recommended dilution range is 1:50 to 1:200. When performing ELISA with a peptide antigen, the appropriate dilution range is usually 1:20000 to 1:40000. It is important to note that these dilution ranges vary depending on the specific experiment and the concentration of the target molecule in the sample. It is crucial to optimize the dilution for each individual experiment to ensure reliable and accurate results.
ImmunogenNotch 1（Cleaved-Val1744）。，。
MW95 kDa
Properties

Concentration

1mg/ml

purificationThe antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
ClonalityPolyclonal Antibody
Stability & StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage bufferRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Target

Background

Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO).

Tissue specificityIn fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.
Posttranslational modificationSynthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by ADAM17 to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). Following endocytosis, this fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.Phosphorylated.O-glycosylated on the EGF-like domains (PubMed:24226769). Contains both O-linked fucose and O-linked glucose in the EGF-like domains 11, 12 and 13, which are interacting with the residues on DLL4 (By similarity). O-linked glycosylation by GALNT11 is involved in determination of left/right symmetry: glycosylation promotes activation of NOTCH1, possibly by promoting cleavage by ADAM17, modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO) (PubMed:24226769).Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH. Monoubiquitination at Lys-1759 is required for activation by gamma-secretase cleavage, it promotes interaction with AAK1, which stabilizes it. Deubiquitination by EIF3F is necessary for nuclear import of activated Notch.Hydroxylated at Asn-1955 by HIF1AN. Hydroxylated at Asn-2022 by HIF1AN (By similarity). Hydroxylation reduces affinity for HI1AN and may thus indirectly modulate negative regulation of NICD (By similarity).
Celluar localizationCytosol;Endoplasmic reticulum;Extracellular region or secreted;Golgi apparatus;Nucleus;Plasma Membrane;
UniPortP46531


Western blot analysis of Notch 1 (Cleaved-Val1744) expression in NIH 3T3 cell extract treated with etoposide

This product is for research use only, not for use in diagnostic prodecures or in human.


Hot Tags: rabbit anti-notch1 (cleaved-val1744) polyclonal antibody#abs131403, China rabbit anti-notch1 (cleaved-val1744) polyclonal antibody#abs131403 suppliers

You Might Also Like

Shopping Bags