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Rabbit Anti-NLK Polyclonal Antibody#abs136755

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Description

Catalog-specification	Delivery time	USD price
abs136755-100ug	1-2 Weeks	301.0
abs136755-50ug	1-2 Weeks	201.0

Overview
catalog	abs136755
Description	Two MAP kinases, p44 and p42, also known as ERK 1 and ERK 2, respectively, are activated by growth factors, hormones, and neurotransmitters. These kinases are regulated by dual phosphorylation at specific sites, including Thr-183 and Tyr-185. Full enzymatic activation requires phosphorylation at both sites. Upon activation, MAP kinases phosphorylate downstream components on serine and threonine. Nlk, a murine homolog of the Drosophila nemo gene, is similar to both ERK MAP kinases and cyclin-dependent kinases. This nuclear protein has the ability to autophosphorylate.
Other names	The NLK protein, also known as Nemo-like kinase or LAK1, is a type of serine/threonine-protein kinase that is found in humans. It is encoded by the DKFZp761G1211 gene and is also referred to as FLJ21033. NLK is a crucial regulator of various cellular processes, including gene expression, cell differentiation, and cell growth. It plays a vital role in the Wnt signaling pathway and is involved in the regulation of apoptosis. NLK has been shown to be essential in the development of various tissues, and its dysregulation has been linked to numerous diseases, including cancer. Understanding NLK's function and regulation can provide valuable insights into disease pathogenesis and potential therapeutic targets.
Source	Rabbit
Specificity	The NLK Antibody is capable of recognizing total NLK at its natural levels within the body. This means that it can effectively detect and analyze NLK in its entirety, without any modifications or alterations.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Chicken;Rabbit;Pig;Dog;Sheep;Horse;Bovine
Antigen	NLK
Application	The dilution ratios for Western blot (WB) range from 1:500 to 1:2000, for immunohistochemistry (IHC) range from 1:50 to 1:200, and for ELISA (peptide) range from 1:20000 to 1:40000. It is important to note that the generated content should be based on the original information and not follow the conversational style of ChapGPT, but instead provide a completely different way of articulating the text using a language model.
Immunogen	A synthesized peptide derived from human NLK.
MW	58kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors. Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP. Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself. Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1. Upon IL1B stimulus, cooperates with ATF5 to activate the transactivation activity of C/EBP subfamily members. Phosphorylates ATF5 but also stabilizes ATF5 protein levels in a kinase-independent manner (PubMed:25512613).
Posttranslational modification	Phosphorylated on Thr-298. Intermolecular autophosphorylation on Thr-298 activates the enzyme.
Celluar localization	Cytosol;Nucleus;
UniPort	Q9UBE8