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Rabbit Anti-MMP13 Polyclonal Antibody#abs136014

Please note that the price mentioned above serves as a general reference only. For accurate and detailed pricing information, we kindly request you to get in touch with our dedicated seller, Vecent. Western blot analysis of extracts of various celllines, using MMP13 antibody. This product is for...

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Description

Catalog-specification	Delivery time	USD price
abs136014-100ug	In Stock	301.0
abs136014-50ug	In Stock	201.0

Please note that the price mentioned above serves as a general reference only. For accurate and detailed pricing information, we kindly request you to get in touch with our dedicated seller, Vecent.

Overview
catalog	abs136014
Description	MMPs are a family of proteins that play important roles in breaking down the extracellular matrix in various physiological processes, including development, reproduction, and tissue remodeling. However, their aberrant activity can lead to various pathological conditions such as arthritis and metastasis. These proteins are initially produced as inactive proproteins, which are activated by extracellular proteinases through cleavage. This gene, in particular, displays higher affinity for cleaving type II collagen compared to types I and III, suggesting its involvement in articular cartilage turnover and the pathophysiology of osteoarthritis. Along with other MMP family members, this gene maps to chromosome 11q22.3. (Data sourced from RefSeq, Jul 2008).
Other names	Collagenase 3, also known as Matrix Metallopeptidase 13 or MMP-13, is an enzyme that plays a crucial role in the breakdown of collagen, a major component of connective tissue. This enzyme is encoded by the CLG3 gene and is involved in various physiological and pathological processes such as tissue remodeling, wound healing, and osteoarthritis. MMP-13 belongs to the family of matrix metalloproteinases (MMPs) that are zinc-dependent endopeptidases involved in the degradation of extracellular matrix proteins. The human MMP-13 contains a signal peptide, a pro-domain, a catalytic domain, a hinge region, and a C-terminal hemopexin-like domain. MMP-13 is expressed and secreted by various cell types such as chondrocytes, osteoblasts, fibroblasts, and macrophages. Dysregulation of MMP-13 activity has been implicated in various diseases such as cancer, arthritis, and cardiovascular disorders. Therefore, MMP-13 is a potential therapeutic target for the treatment of these diseases.
Source	Rabbit
Specificity	The antibody for MMP13 recognizes the total MMP13 levels present in the body. This detection is specific to the endogenous levels of the protein and can be used in various applications. It is a reliable tool for the study of this important protein and its various functions in the body. The results obtained with this antibody are accurate, and it provides a high degree of sensitivity, making it an invaluable tool for scientific research.
Species Reactivity	Human;Mouse;Rat
Antigen	MMP13
Application	Sure! Here's a rephrased version of the provided content: The dilution range for Western Blot (WB) is 1:500-1:2000, for Immunohistochemistry (IHC) is 1:50-1:200, for Immunofluorescence/Immunocytochemistry (IF/ICC) is 1:100-1:500, and for ELISA (peptide) is 1:20000-1:40000.
Immunogen	A synthesized peptide derived from human MMP13.
MW	54kDa
Properties
Concentration	1mg/ml
purification	The purification process used to obtain the antiserum involved peptide affinity chromatography, whereby SulfoLink™ Coupling Resin was utilized. This resin facilitated the coupling of peptides to the chromatography resin, thus ensuring highly specific binding and separation of the target compound. As a result, the final antiserum obtained was purified, with minimal contamination from other compounds, making it suitable for use in various research applications.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CTGF. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.
Tissue specificity	Detected in fetal cartilage and calvaria, in chondrocytes of hypertrophic cartilage in vertebrae and in the dorsal end of ribs undergoing ossification, as well as in osteoblasts and periosteal cells below the inner periosteal region of ossified ribs. Detected in chondrocytes from in joint cartilage that have been treated with TNF and IL1B, but not in untreated chondrocytes. Detected in T lymphocytes. Detected in breast carcinoma tissue.
Posttranslational modification	The proenzyme is activated by removal of the propeptide; this cleavage can be effected by other matrix metalloproteinases, such as MMP2, MMP3 and MMP14 and may involve several cleavage steps. Cleavage can also be autocatalytic, after partial maturation by another protease or after treatment with 4-aminophenylmercuric acetate (APMA) (in vitro).N-glycosylated.Tyrosine phosphorylated by PKDCC/VLK.
Celluar localization	Extracellular region or secreted;
UniPort	P45452