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Rabbit Anti-MaxiKbeta Polyclonal Antibody#abs138091

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Description

Catalog-specification	Delivery time	USD price
abs138091	1-2 Weeks	201
abs138091	1-2 Weeks	301

Overview
Description
Other names	The beta 4 subunit of the BK channel, also known as BK channel subunit beta-4 or BKbeta4, plays a crucial role in calcium-activated potassium channels. It is involved in regulating the flow of potassium ions in response to changes in intracellular calcium levels. This subunit is also referred to as the calcium-activated potassium channel subfamily M subunit beta 4 or simply the calcium-activated potassium channel subunit beta 4. Another name for the beta 4 subunit is the charybdotoxin receptor subunit beta 4, highlighting its role in binding to charybdotoxin, a toxin that specifically targets potassium channels. It is part of the K(VCA)beta 4 or K(VCA)beta-4 family of potassium channels. In humans, the beta 4 subunit is encoded by the KCNMB4 gene. It forms a complex with other subunits, contributing to the formation of the large conductance calcium-dependent potassium ion channel, also known as the Maxi K channel. This channel is essential for various physiological processes, including muscle relaxation, neuronal excitability regulation, and blood vessel dilation. Understanding the function and characteristics of the beta 4 subunit can provide insights into the mechanisms underlying calcium-activated potassium channels and their role in various physiological and pathological conditions.
Source	Rabbit
Specificity	The detection of endogenous levels of total MaxiKβ can be achieved using the MaxiKβ Antibody. By identifying and measuring the presence of MaxiKβ, this antibody allows for the accurate assessment of its levels within the biological system. This particular antibody is highly effective in recognizing the native form of MaxiKβ, enabling researchers to study its role and function in various physiological processes. With its ability to detect endogenous levels, the MaxiKβ Antibody has proven to be a valuable tool in understanding the regulatory mechanisms and potential therapeutic implications associated with this protein.
Species Reactivity	Human;Mouse;Rat;Monkey
Predictive reaction species	Pig;Sheep;Bovine;Rabbit;
Antigen	MaxiKβ
Application	The recommended dilution for Western blotting ranges from 1:1000 to 3000, while the ideal dilution for immunohistochemistry is 1:200. For ELISA assays using peptide, the suggested dilution ranges from 1:20000 to 1:40000. It is important to note that these dilutions may need to be optimized based on the specific experimental conditions and target protein concentration.
Immunogen	A synthesized peptide derived from human MaxiKβ.
MW	23 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum was purified via peptide affinity chromatography. It was an effective method of purification that resulted in a high-quality end product.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure optimal storage, it is recommended that the product be kept at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles as this may impact its effectiveness and quality. As such, it is crucial to take the necessary precautions to maintain the integrity of the product during storage.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Decreases the gating kinetics and calcium sensitivity of the KCNMA1 channel, but with fast deactivation kinetics. May decrease KCNMA1 channel openings at low calcium concentrations but increases channel openings at high calcium concentrations. Makes KCNMA1 channel resistant to 100 nM charybdotoxin (CTX) toxin concentrations.
Tissue specificity	Predominantly expressed in brain. In brain, it is expressed in the cerebellum, cerebral cortex, medulla, spinal cord, occipital pole, frontal lobe, temporal lobe, putamen, amygdala, caudate nucleus, corpus callosum, hippocampus, substantia nigra and thalamus. Weakly or not expressed in other tissues.
Posttranslational modification	Phosphorylated. Phosphorylation modulates its effect on KCNMA1 activation kinetics.N-glycosylated. A highly glycosylated form is promoted by KCNMA1. Glycosylation, which is not required for the interaction with KCNMA1 and subcellular location, increases protection against charybdotoxin.
Celluar localization	Plasma Membrane;
UniPort	Q86W47