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Rabbit Anti-MAD1L1 Polyclonal Antibody#abs135807

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Description

Catalog-specification	Delivery time	USD price
abs135807-100ug	1-2 Weeks	301.0
abs135807-50ug	1-2 Weeks	201.0

Overview
catalog	abs135807
Description	MAD1L1 is a vital component of the mitotic spindle-assembly checkpoint, crucial for ensuring that the onset of anaphase does not occur until all chromosomes are correctly aligned at the metaphase plate. This important protein functions as a homodimer, and its role is enhanced by its interaction with MAD2L1. Cell cycle control and tumor suppression may both be part of MAD1L1's roles. This gene has been found to have three transcript variants that all encode the same protein. This information has been provided by RefSeq, as of July 2008.
Other names	MAD1 is a critical player in mitotic spindle assembly checkpoint pathways, which has several other aliases. Some of these aliases include HsMAD1, MAD1-like protein 1, and Mitotic checkpoint MAD1 protein homolog. Additionally, MAD1 has been identified as a tax-binding protein 181 and TP53I9, which is a tumor protein p53 inducible protein. Notably, MAD1 has homologs in yeast which are responsible for mitotic arrest deficiencies. Another important facet of MAD1 is its ability to bind to the Tax oncoprotein, which has implications in cancer research. However, MAD1's primary function lies in the regulation of the mitotic spindle assembly checkpoint, which ensures that the cell's genetic material is correctly segregated during cell division. As a key component of this pathway, MAD1 works in concert with other checkpoint proteins to prevent the cell from progressing through mitosis until all chromosomes have properly attached to the spindle. Dysregulation of MAD1 or other checkpoint proteins can lead to chromosomal instability and tumorigenesis.
Source	Rabbit
Specificity	The MAD1L1 Antibody is capable of identifying the presence of total MAD1L1 at its natural levels within the body. By rearranging the given information, a similar content could be generated, providing insights into the antibody's ability to detect endogenous MAD1L1 levels.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Bovine;Horse;Pig
Antigen	MAD1L1
Application	The dilutions for western blot (WB) range from 1:500 to 1:2000, for immunohistochemistry (IHC) range from 1:50 to 1:200, and for ELISA (peptide) range from 1:20000 to 1:40000. It is important to note that the dilutions vary depending on the technique used.
Immunogen	A synthesized peptide derived from human MAD1L1.
MW	83kDa
Properties
Concentration	1mg/ml
purification	Using the SulfoLink™ Coupling Resin, the antiserum underwent a purification process via peptide affinity chromatography, resulting in its enhanced quality.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.
Tissue specificity	Expressed weakly at G0/G1 and highly at late S and G2/M phase.
Posttranslational modification	Phosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage.
Celluar localization	Cytoskeleton;Cytosol;Nucleus;
UniPort	Q9Y6D9