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Rabbit Anti-AMBRA1 Polyclonal Antibody#abs135748

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Description

Catalog-specification	Delivery time	USD price
abs135748-100ug	1-2 Weeks	301.0
abs135748-50ug	1-2 Weeks	201.0

Overview
catalog	abs135748
Description	One common motif found in a variety of proteins are WD-repeats, which are characterized by a conserved core of 40-60 amino acids forming a tertiary propeller structure. These proteins play a role in a wide range of cellular functions such as chromatin assembly, cell cycle control, and RNA processing. Another protein containing WD-repeats is AMBRA1, also known as WDR94 or KIAA1736. This 1,298 amino acid protein is localized to cytoplasmic vesicles and functions in controlling protein turnover, cell proliferation, and cell survival during neuronal development. AMBRA1 plays a vital role in autophagy and the development of the nervous system. Due to alternative splicing events, multiple isoforms of AMBRA1 exist.
Other names	The protein Activating molecule in beclin 1 regulated autophagy is also known as AMBRA1_HUMAN, BECN1-regulated autophagy protein 1, FLJ20294, Hypothetical protein FLJ20294, Hypothetical protein LOC55626, KIAA1736, MGC33725, and WDR94. This protein plays a crucial role in the autophagy process which is responsible for degrading and recycling damaged or unnecessary cellular components. The activation of BECN1 regulated autophagy requires the participation of Activating molecule in BECN1-regulated autophagy protein 1. This protein is also involved in many other cellular processes, making it an essential component of intracellular signaling pathways. Its study is crucial for understanding the mechanisms of cell homeostasis and cellular response to environmental changes.
Source	Rabbit
Specificity	The total levels of AMBRA1 can be detected by the AMBRA1 antibody, which specifically targets the endogenous AMBRA1 protein. To elaborate, this antibody is designed to recognize and bind to the native AMBRA1 present in cells or tissues. By using this antibody, researchers can accurately measure the overall expression levels of AMBRA1 in biological samples.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Rabbit;Sheep;Dog;Horse;Bovine;
Antigen	AMBRA1
Application	The dilution ratios for Western blotting (WB) range from 1:500 to 1:2000. For immunohistochemistry (IHC), the suitable dilution range is 1:50 to 1:200. The peptide ELISA requires a dilution range of 1:20000 to 1:40000. It is important to note that the generated content will be based on the original text information and will not follow the structure and style of ChapGPT-generated content.
Immunogen	A synthesized peptide derived from human AMBRA1.
MW	133kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation (By similarity).
Celluar localization	Cytoskeleton;Cytosol;Mitochondrion;
UniPort	Q9C0C7