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Rabbit Anti-IL17A Polyclonal Antibody#abs135647

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Description

Catalog-specification	Delivery time	USD price
abs135647-100ug	1-2 Weeks	301.0
abs135647-50ug	1-2 Weeks	201.0

Overview
catalog	abs135647
Description	IL-17A is a pro-inflammatory cytokine produced by a distinct CD4+ T cell lineage known as Th17 cells. This cystine-linked homodimeric cytokine has been shown to stimulate the production of other pro-inflammatory cytokines like TNF-α, IL-1β, and IL-6. IL-17A also plays a vital role in innate immunity by inducing the production of neutrophil chemoattractants like CXCL1, CXCL6, and IL-8. Interestingly, this cytokine is also involved in mucosal immunity particularly against bacterial infections, but it has also been implicated in some autoimmune disorders. IL-17A's effects are primarily exerted through its binding to the IL-17RA receptor. This binding triggers the activation of several pathways including the Erk1/2 MAP kinase, PI3K/Akt, p38, and NF-κB pathways. Activated pathways lead to the production of an array of cytokines, chemokines, and other proteins, thus playing a significant role in immune regulation. Some Jaks and Stats have also been observed to undergo phosphorylation in response to IL-17A signaling.
Other names	CTLA-8, also known as Cytotoxic T lymphocyte associated antigen 8 or Cytotoxic T lymphocyte associated protein 8, is a protein involved in immune responses. It is also referred to as Cytotoxic T lymphocyte associated serine esterase 8 or Cytotoxic T-lymphocyte-associated antigen 8. Another name for this protein is Interleukin 17A (IL-17A), which is part of the Interleukin-17 (IL-17) family. IL-17A plays a role in inflammation and immune regulation. It is encoded by the IL17A gene and is sometimes denoted as IL17 or IL17A. In mice, the corresponding gene is referred to as Il17a. These names and acronyms are used to describe the same protein molecule.
Source	Rabbit
Specificity	The IL17A Antibody is capable of detecting total IL17A levels in the body. Its function is to recognize and bind to endogenous forms of IL17A. This antibody is highly useful in research studies where detecting IL17A is critical. It accurately measures the endogenous levels of this protein for accurate analysis and interpretation of experimental data. By targeting endogenous forms of IL17A, the IL17A Antibody is a valuable tool for understanding the function and role of this protein in the body.
Species Reactivity	Human;Mouse;Rat
Antigen	IL17A
Application	The recommended dilution range for primary antibodies varies depending on the application. For Western blot analysis, dilutions of 1:200 to 1:500 are suggested. When performing immunohistochemistry, dilutions of 1:50 to 1:200 are typically used. For immunofluorescence assays, dilutions of 1:20 to 1:50 are appropriate. ELISA assays using a peptide substrate typically require higher dilution ranges, with suggested dilutions ranging from 1:20,000 to 1:40,000. Regardless of the application, it is important to optimize dilutions for each antibody to ensure optimal performance.
Immunogen	A synthesized peptide derived from human IL17A.
MW	18kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Ligand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines (PubMed:8676080).
Tissue specificity	Restricted to activated memory T-cells.
Posttranslational modification	Found both in glycosylated and nonglycosylated forms.
Celluar localization	Extracellular region or secreted;Plasma Membrane;
UniPort	Q16552