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Rabbit Anti-IKBKG Polyclonal Antibody#abs135663

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Description

Catalog-specification	Delivery time	USD price
abs135663-100ug	1-2 Weeks	301.0
abs135663-50ug	1-2 Weeks	201.0

Overview
catalog	abs135663
Description	The inactive NF-κB/Rel transcription factors are bound to IκB proteins within the cytosol (1-3). The majority of stimuli that activate NF-κB initiate a common pathway involving proteasome-mediated degradation of IκB by phosphorylation (3-7). This pathway's critical regulation step involves the activation of the IKK complex, a high molecular weight kinase complex composed of three tightly associated IKK subunits. IKKα, IKKβ, and IKKγ serve as the catalytic and regulatory subunits of the kinase, respectively (8,9). IKK activation is prompted by the phosphorylation of Ser177 and Ser181 in the activation loop of IKKβ (or Ser176 and Ser180 in IKKα). Consequently, the phosphorylation causes structural changes resulting in kinase activation (10-13).
Other names	IkB kinase associated protein 1, also known as IkB kinase subunit gamma or inhibitor of nuclear factor kappa B kinase subunit gamma, plays a vital role in regulating the immune response. It is encoded by the IKBKG gene and is also known by several other names such as AMCBX1, FIP 3, FIP-3, FIP3, Fip3p, I kappa B kinase gamma and IKK-gamma. This protein is a key component of the NF-kappaB signaling pathway that controls a variety of cellular processes, including cell growth, proliferation, and survival. NEMO (NF kappa B essential modifier) is another name for IkB kinase associated protein 1 or IKK-gamma. The protein is encoded by the IKBKG gene and is required for the activation of NF-kappaB transcription factors. NEMO plays a crucial role in the immune system, as it regulates the expression of genes associated with inflammation, immunity, and cancer. Additionally, mutations in the IKBKG gene can lead to the development of diseases such as incontinentia pigmenti, a rare genetic disorder that affects the skin, hair, teeth, and nails. In summary, IkB kinase associated protein 1 or NEMO is a crucial component of the NF-kappaB signaling pathway that regulates immune responses and controls cellular processes, such as cell growth, proliferation, and survival. It is also known by several other names, including I-kappa-B kinase subunit gamma and NF-kappa-B essential modifier. Mutations in the IKBKG gene can lead to the development of various diseases, including incontinentia pigmenti.
Source	Rabbit
Specificity	The endogenous levels of total IKBKG can be detected by the IKBKG Antibody. By rearranging the original content, a similar statement can be generated.
Species Reactivity	Human;Mouse;Rat
Antigen	IKBKG
Application	For Western blotting, dilutions of 1:500 to 1:2000 are recommended. For immunohistochemistry, dilutions of 1:50 to 1:200 are suitable. For immunofluorescence and immunocytochemistry, dilutions of 1:100 to 1:500 are suggested. When performing an ELISA using a peptide, a recommended dilution range of 1:20000 to 1:40000 is recommended. It is important to use appropriate dilutions in order to obtain optimal results for each application.
Immunogen	A synthesized peptide derived from human IKBKG.
MW	48kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. However, the specific type of polyubiquitin recognized upon cell stimulation (either 'Lys-63'-linked or linear polyubiquitin) and its functional importance is reported conflictingly. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination.
Tissue specificity	Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Posttranslational modification	Phosphorylation at Ser-68 attenuates aminoterminal homodimerization.Polyubiquitinated on Lys-285 through 'Lys-63'; the ubiquitination is mediated by NOD2 and RIPK2 and probably plays a role in signaling by facilitating interactions with ubiquitin domain-containing proteins and activates the NF-kappa-B pathway. Polyubiquitinated on Lys-399 through 'Lys-63'; the ubiquitination is mediated by BCL10, MALT1 and TRAF6 and probably plays a role in signaling by facilitating interactions with ubiquitin domain-containing proteins and activates the NF-kappa-B pathway. Monoubiquitinated on Lys-277 and Lys-309; promotes nuclear export. Polyubiquitinated through 'Lys-27' by TRIM23; involved in antiviral innate and inflammatory responses. Linear polyubiquitinated on Lys-111, Lys-143, Lys-226, Lys-246, Lys-264, Lys-277, Lys-285, Lys-292, Lys-302, Lys-309 and Lys-326; the head-to-tail polyubiquitination is mediated by the LUBAC complex and plays a key role in NF-kappa-B activation. Polyubiquitinated on Lys-309 and Lys-321 via 'Lys-27'-linked ubiquitin by Shigella flexneri E3 ubiquitin-protein ligase ipah9.8, leading to its degradation by the proteasome. Deubiquitinated by USP10 in a TANK-dependent and -independent manner, leading to the negative regulation of NF-kappaB signaling upon DNA damage (PubMed:25861989).Sumoylated on Lys-277 and Lys-309 with SUMO1; the modification results in phosphorylation of Ser-85 by ATM leading to a replacement of the sumoylation by mono-ubiquitination on these residues.Neddylated by TRIM40, resulting in stabilization of NFKBIA and down-regulation of NF-kappa-B activity.
Celluar localization	Cytoskeleton;Cytosol;Nucleus;
UniPort	Q9Y6K9