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Rabbit Anti-HNF4A Polyclonal Antibody#abs136329

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Description

Catalog-specification	Delivery time	USD price
abs136329-100ug	1-2 Weeks	301.0
abs136329-50ug	1-2 Weeks	201.0

Overview
catalog	abs136329
Description	HNF4α, also known as hepatocyte nuclear factor 4α, is a transcription factor that is primarily found in the liver and belongs to the steroid hormone receptor superfamily (1). Its role in regulating gluconeogenic genes, such as phosphoenolpyruvate carboxykinase and glucose-6-phosphatase, is enhanced when it forms a complex with PGC-1α, particularly in fasted livers (2,3). Interestingly, when the HNF4α gene is conditionally knocked out in the liver of mice, it disrupts lipid homeostasis, leading to the accumulation of lipids in the liver and a decrease in serum cholesterol and triglyceride levels (4). Remarkably, mutations in the HNF4α gene have been associated with maturity-onset diabetes of the young (MODY), further highlighting its crucial role in regulating glucose metabolism and overall metabolic health (5).
Other names	Hepatic nuclear factor 4 alpha, also known as HNF 4 alpha or HNF4A, is a transcription factor that plays a vital role in regulating gene expression in the liver. It is part of the nuclear receptor subfamily 2 group A and is involved in various biological processes, including glucose metabolism and lipid homeostasis. HNF 4 alpha, or hepatocyte nuclear factor 4 alpha, is encoded by the HNF4A gene and is a key player in the development and function of hepatocytes, the main functional cells of the liver. It controls the expression of numerous genes involved in liver-specific functions, such as detoxification, nutrient metabolism, and bile acid synthesis. Mutations in the HNF4A gene can lead to a monogenic form of diabetes called Maturity-Onset Diabetes of the Young (MODY), specifically MODY1. MODY is characterized by early-onset diabetes and impaired insulin production. HNF 4 alpha consists of multiple isoforms, including HNF4a7, HNF4a8, and HNF4a9. Each isoform has slightly different functions and expression patterns. Overall, HNF 4 alpha is a critical transcription factor that orchestrates gene expression in the liver, influencing various metabolic pathways and maintaining liver health. Understanding its role and regulation is important for elucidating the molecular mechanisms underlying liver diseases and metabolic disorders.
Source	Rabbit
Specificity	The HNF4A Antibody is capable of detecting the overall levels of endogenous HNF4A. This antibody works by recognizing and binding with the protein, providing insight into the quantity of HNF4A present in a given sample. It should be noted that this antibody is highly specific and can effectively detect total HNF4A levels. Overall, the HNF4A Antibody is a valuable tool for researchers seeking to study the function and expression of HNF4A protein in various tissues and cell types.
Species Reactivity	Human;Mouse;Rat
Antigen	HNF4A
Application	。Western Blotting (WB)，1:5001:2000。（IHC），1:501:200。/（IF/ICC），1:1001:500。（ELISA），1:200001:40000。，，，。
Immunogen	A synthetic peptideof human HNF4A.
MW	52kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine.
Posttranslational modification	Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA.Acetylation at Lys-458 lowers transcriptional activation by about two-fold.
Celluar localization	Nucleus;
UniPort	P41235