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Rabbit Anti-HIPK2 Polyclonal Antibody#abs137459

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Description

Catalog-specification	Delivery time	USD price
abs137459-100ug	1-2 Weeks	301.0
abs137459-50ug	1-2 Weeks	201.0

Overview
catalog	abs137459
Other names	HIPK2, also known as Homeodomain interacting protein kinase 2 or HIPK2_HUMAN, is a crucial protein involved in various cellular processes. It is encoded by the Nbak1 gene and is sometimes referred to as Nuclear body-associated kinase 1 or PRO0593. Another name for HIPK2 is Sialophorin tail-associated nuclear serine/threonine-protein kinase. This protein, also known as Stank, plays a significant role in mediating cellular signaling pathways and protein interactions. Its importance lies in its ability to regulate gene expression and control cell growth, differentiation, and survival. HIPK2 is a multifunctional protein that is involved in the regulation of several key cellular processes, making it an intriguing area of study for researchers.
Source	Rabbit
Specificity	The HIPK2 Antibody has the ability to detect the total endogenous levels of HIPK2. This signifies that the antibody is useful in studying the expression and function of HIPK2 in various biological processes. By detecting the endogenous levels of HIPK2, the antibody provides insights into the role of HIPK2 in disease development and the identification of new therapeutic targets. Overall, this antibody is an important tool for researchers studying the functions of HIPK2.
Species Reactivity	Human;Mouse
Predictive reaction species	Bovine;Horse;
Antigen	HIPK2
Application	The suggested dilution ranges for Western Blot (WB) are 1:1000-3000, for Immunohistochemistry (IHC) are 1:200, and for ELISA (peptide) are 1:20000-1:40000. Please note that the generated content will be based on the original information but rearranged in a different format.
Immunogen	A synthesized peptide derived from human HIPK2.
MW	131 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent purification via peptide affinity chromatography. To ensure high-quality results, this process was carefully carried out with great attention to detail. The end result was a purified antiserum with elevated levels of efficacy and purity, making it a valuable addition to any laboratory's toolkit.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve the quality of the product, it is recommended to store it at a temperature of -20 °C for up to one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles, which can compromise its integrity and effectiveness. Be sure to follow the storage instructions closely to ensure maximum potency and longevity.
Storage buffer	To ensure the long-term stability of Rabbit IgG, it is recommended to store it at a temperature of -20 °C. The IgG is formulated in phosphate buffered saline with a pH of 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. This storage condition allows the IgG to remain stable for a period of 12 months starting from the date of its receipt.
Target
Background	Serine/threonine-protein kinase involved in transcription regulation, p53/TP53-mediated cellular apoptosis and regulation of the cell cycle. Acts as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. Phosphorylates PDX1, ATF1, PML, p53/TP53, CREB1, CTBP1, CBX4, RUNX1, EP300, CTNNB1, HMGA1 and ZBTB4. Inhibits cell growth and promotes apoptosis through the activation of p53/TP53 both at the transcription level and at the protein level (by phosphorylation and indirect acetylation). The phosphorylation of p53/TP53 may be mediated by a p53/TP53-HIPK2-AXIN1 complex. Involved in the response to hypoxia by acting as a transcriptional co-suppressor of HIF1A. Mediates transcriptional activation of TP73. In response to TGFB, cooperates with DAXX to activate JNK. Negative regulator through phosphorylation and subsequent proteasomal degradation of CTNNB1 and the antiapoptotic factor CTBP1. In the Wnt/beta-catenin signaling pathway acts as an intermediate kinase between MAP3K7/TAK1 and NLK to promote the proteasomal degradation of MYB. Phosphorylates CBX4 upon DNA damage and promotes its E3 SUMO-protein ligase activity. Activates CREB1 and ATF1 transcription factors by phosphorylation in response to genotoxic stress. In response to DNA damage, stabilizes PML by phosphorylation. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation. Promotes angiogenesis, and is involved in erythroid differentiation, especially during fetal liver erythropoiesis. Phosphorylation of RUNX1 and EP300 stimulates EP300 transcription regulation activity. Triggers ZBTB4 protein degradation in response to DNA damage. Modulates HMGA1 DNA-binding affinity. In response to high glucose, triggers phosphorylation-mediated subnuclear localization shifting of PDX1. Involved in the regulation of eye size, lens formation and retinal lamination during late embryogenesis.
Tissue specificity	Highly expressed in heart, muscle and kidney. Weakly expressed in a ubiquitous way. Down-regulated in several thyroid and breast tumors.
Posttranslational modification	Autophosphorylation at Tyr-361 in the activation loop activates the kinase and promotes nuclear localization.Sumoylated. When conjugated it is directed to nuclear speckles. Desumoylated by SENP1 (By similarity). Sumoylation on Lys-32 is promoted by the E3 SUMO-protein ligase CBX4.Ubiquitinated by FBXO3, WSB1 and SIAH1, leading to rapid proteasome-dependent degradation. The degradation mediated by FBXO3, but not ubiquitination, is prevented in the presence of PML. The degradation mediated by WSB1 and SIAH1 is reversibly reduced upon DNA damage.Cleaved at Asp-923 and Asp-984 by CASP6 in a p53/TP53-dependent manner. The cleaved form lacks the autoinhibitory C-terminal domain (AID), resulting in a hyperactive kinase, which potentiates p53/TP53 Ser-46 phosphorylation and subsequent activation of the cell death machinery.
Celluar localization	Nucleus;
UniPort	Q9H2X6