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Rabbit Anti-HIF1AN Polyclonal Antibody#abs136866

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Description

Catalog-specification	Delivery time	USD price
abs136866-100ug	1-2 Weeks	301.0
abs136866-50ug	1-2 Weeks	201.0

Overview
catalog	abs136866
Other names	FIH-1, also known as Factor inhibiting HIF-1 or Hypoxia-inducible factor 1 alpha inhibitor, is a protein involved in the regulation of HIF-1 (Hypoxia-inducible factor 1). Other names for FIH-1 include FIH1, FLJ20615, FLJ22027, HIF1AN, HIF1N_HUMAN, Hypoxia inducible factor 1 alpha subunit inhibitor, and Peptide aspartate beta dioxygenase. FIH-1 acts as an inhibitor of HIF-1 by hydroxylating specific asparagine residues in the HIF-1 alpha subunit. This hydroxylation prevents the interaction of HIF-1 with coactivators and subsequently inhibits the transcriptional activation of genes involved in cellular responses to hypoxia. Overall, FIH-1 plays a critical role in the regulation of HIF-1 and the cellular response to low oxygen levels.
Source	Rabbit
Specificity	The antibody HIF1AN is capable of detecting the total amount of endogenous HIF1AN protein present in a sample. To ensure accurate results, it is essential to use a highly similar content rearranged from the original information.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Xenopus;Rabbit;Pig;Dog;Sheep;Bovine;Horse
Antigen	HIF1AN
Application	IHC: The dilution range for WB is 1:500-1:2000, while for IHC it is 1:50-1:200. In ELISA tests using peptides, the recommended dilution is 1:20000-1:40000. It is important to note that these dilution ratios are based on the original information provided.
Immunogen	A synthesized peptide derived from human HIF1AN.
MW	40kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, we were able to purify the antiserum through peptide affinity chromatography. Our process involved careful selection and binding of specific peptides to the resin in order to capture and isolate the desired antibody. The resulting purified antiserum is highly specific and effective, making it a valuable tool for research and medical applications.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality, it is recommended to store the product at -20 °C for a period of one year. It is important to avoid subjecting it to multiple freeze/thaw cycles.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.
Celluar localization	Cytosol;Nucleus;
UniPort	Q9NWT6