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Rabbit Anti-GSK3B Polyclonal Antibody#abs136743

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Description

Catalog-specification	Delivery time	USD price
abs136743-50ug	1-2 Weeks	201.0
abs136743-100ug	1-2 Weeks	301.0

Overview
catalog	abs136743
Description	The gene in question codes for a type of kinase called serine-threonine kinase. It is classified as part of the glycogen synthase kinase subfamily and is known to play a role in a variety of biological processes including energy metabolism, neuronal cell development, and body pattern formation. There is evidence to suggest that certain variations in this gene may increase or decrease the risk of developing Parkinson disease. Additionally, studies conducted in mice have shown that increased expression of this gene may be related to the development of Alzheimer disease. Multiple variants of this gene, which encode different protein isoforms, have been identified through alternative splicing.
Other names	The protein known as Glycogen Synthase Kinase 3 Beta, or GSK 3 beta, is an important enzyme that serves essential functions in many biological processes. This serine/threonine-protein kinase is a key regulator of glycogen synthesis and plays a major role in the regulation of glucose metabolism. GSK-3 beta is also involved in neuronal signaling and has been implicated in numerous diseases including Alzheimer's disease, diabetes, and cancer. GSK3B is the human isoform of this protein and mutations in this gene have been linked to various diseases and disorders. There is a great deal of interest in understanding the precise molecular mechanisms that underlie the activities of GSK-3 beta, as it is believed that targeting this enzyme may hold promise for the development of new treatments for a variety of health conditions. Overall, GSK 3 beta is a fascinating protein with a wide range of functions and is the focus of intense research efforts in the scientific community.
Source	Rabbit
Specificity	The presence of total GSK3B levels can be accurately determined using the GSK3B Antibody, which is specifically designed to detect endogenous levels of the protein.
Species Reactivity	Human;Mouse;Rat
Antigen	GSK3B
Application	The recommended dilution for WB is 1:500-1:2000, for IF/ICC it is 1:100-1:500, and for ELISA (peptide) it is 1:20000-1:40000. These dilutions ensure optimal results when using the respective techniques. It is important to note that dilution may vary depending on the sample type and antigen concentration. Therefore, it is recommended to perform a titration experiment to determine the optimal dilution for specific experiments.
Immunogen	A synthetic peptide of human GSK3B.
MW	48kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized for peptide affinity chromatography to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates. In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. May also mediate the development of insulin resistance by regulating activation of transcription factors. Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase. In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates NFATC1/NFATC on conserved serine residues promoting NFATC1/NFATC nuclear export, shutting off NFATC1/NFATC gene regulation, and thereby opposing the action of calcineurin. Phosphorylates MAPT/TAU on 'Thr-548', decreasing significantly MAPT/TAU ability to bind and stabilize microtubules. MAPT/TAU is the principal component of neurofibrillary tangles in Alzheimer disease. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Phosphorylates MACF1, inhibiting its binding to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. Probably regulates NF-kappa-B (NFKB1) at the transcriptional level and is required for the NF-kappa-B-mediated anti-apoptotic response to TNF-alpha (TNF/TNFA). Negatively regulates replication in pancreatic beta-cells, resulting in apoptosis, loss of beta-cells and diabetes. Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation. Phosphorylates MUC1 in breast cancer cells, decreasing the interaction of MUC1 with CTNNB1/beta-catenin. Is necessary for the establishment of neuronal polarity and axon outgrowth. Phosphorylates MARK2, leading to inhibit its activity. Phosphorylates SIK1 at 'Thr-182', leading to sustain its activity. Phosphorylates ZC3HAV1 which enhances its antiviral activity. Phosphorylates SNAI1, leading to its BTRC-triggered ubiquitination and proteasomal degradation. Phosphorylates SFPQ at 'Thr-687' upon T-cell activation. Phosphorylates NR1D1 st 'Ser-55' and 'Ser-59' and stabilizes it by protecting it from proteasomal degradation. Regulates the circadian clock via phosphorylation of the major clock components including ARNTL/BMAL1, CLOCK and PER2. Phosphorylates CLOCK AT 'Ser-427' and targets it for proteasomal degradation. Phosphorylates ARNTL/BMAL1 at 'Ser-17' and 'Ser-21' and primes it for ubiquitination and proteasomal degradation. Phosphorylates OGT at 'Ser-3' or 'Ser-4' which positively regulates its activity. Phosphorylates MYCN in neuroblastoma cells which may promote its degradation (PubMed:24391509).
Tissue specificity	Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. Colocalizes with EIF2AK2/PKR and TAU in the Alzheimer disease (AD) brain.
Posttranslational modification	Phosphorylated by AKT1 and ILK1. Upon insulin-mediated signaling, the activated PKB/AKT1 protein kinase phosphorylates and desactivates GSK3B, resulting in the dephosphorylation and activation of GYS1. Activated by phosphorylation at Tyr-216 (PubMed:25169422). Inactivated by phosphorylation at Ser-9 (Probable).Mono-ADP-ribosylation by PARP10 negatively regulates kinase activity.
Celluar localization	Cytoskeleton;Cytosol;Mitochondrion;Nucleus;Plasma Membrane;
UniPort	P49841