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Rabbit Anti-Estrogen Receptor-beta Polyclonal Antibody#abs131955

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Description

Catalog-specification	Delivery time	USD price
abs131955-100ug	1-2 Weeks	301.0
abs131955-50ug	1-2 Weeks	201.0

Overview
catalog	abs131955
Description	ESR2, known as estrogen receptor-beta, belongs to the nuclear receptor superfamily. It possesses the ability to convert external signals into transcriptional responses.
Other names	ER beta, also known as ER-beta or ESR2, is a member of the estrogen receptor family that plays important roles in various physiological processes in the body. This receptor has several variants such as Erb, ESR B, ESR BETA, ESR2 HUMAN, ESRB, ESTRB, estrogen nuclear receptor beta variant a, estrogen nuclear receptor beta variant b, estrogen receptor 2 (ER beta), estrogen receptor beta 4, and Nuclear receptor subfamily 3 group A member 2. ER beta is found in various tissues such as the reproductive tract, breast, bone, cardiovascular system, and nervous system. Its main function is to bind to estrogen and regulate gene expression, leading to the development and maintenance of these tissues. ER beta is also involved in modulating the immune response, metabolism, and cognitive function. ER beta has been studied extensively in the context of breast cancer. Studies have shown that ER beta can inhibit the growth of breast cancer cells and enhance the effectiveness of chemotherapy and hormone therapy. ER beta has also been implicated in the development of other types of cancer, such as prostate and colon cancer. Overall, ER beta is a crucial receptor in the body that plays diverse roles in physiology and pathology. Its variants and localization suggest that its function is context-dependent and requires further investigation.
Source	Rabbit
Specificity	This antibody is specifically designed to recognize and bind to endogenous levels of total Estrogen Receptor-beta, enabling researchers to study its functions and activities. By using this antibody, researchers can gain insights into the role of Estrogen Receptor-beta in various physiological and pathological processes.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Horse;Dog;
Antigen	Estrogen Receptor-β
Application	The recommended dilutions for Western blotting (WB) range from 1:500 to 1:2000. For immunohistochemistry (IHC), the suggested dilutions are between 1:50 and 1:200. In the case of immunofluorescence or immunocytochemistry (IF/ICC), dilutions of 1:100 to 1:500 are advised. Lastly, for ELISA using peptides, dilutions of 1:20000 to 1:40000 are recommended. Please note that these dilutions are based on the original information provided and aim to assist you in your experimental applications.
Immunogen	A peptide with a composition originating from Estrogen Receptor-beta in humans has been synthesized. Let's generate a highly similar version of the content by rearranging the provided information. Please note that I will not rely on ChapGPT's way of generating content, but rather employ a distinct approach to deliver the speech.
MW	59kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized to perform peptide affinity chromatography for the purification of the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560). Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
Tissue specificity	Isoform 1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform 2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform 4 is found in testis. Isoform 5 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform 6 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.
Posttranslational modification	Phosphorylation at Ser-87 and Ser-105 recruits NCOA1.
Celluar localization	Extracellular region or secreted;Mitochondrion;Nucleus;
UniPort	Q92731

Western blot analysis of Estrogen Receptor-beta expression in EGF treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

This product is for research use only, not for use in diagnostic prodecures or in human.

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