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Rabbit Anti-EIF3E Polyclonal Antibody#abs137927

Description

Catalog-specification	Delivery time	USD price
abs137927-100ug	1-2 Weeks	301.0
abs137927-50ug	1-2 Weeks	201.0

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Overview
catalog	abs137927
Other names	EIF3E, also known as eIF-3 p48 or eIF3S6, is a subunit of the eukaryotic translation initiation factor 3 (eIF3). This protein, encoded by the EIF3E_HUMAN gene, plays a crucial role in the initiation of protein synthesis in eukaryotic cells. It is part of a multiprotein complex that helps to assemble the ribosome and promotes the binding of the initiator tRNA to the mRNA. The eIF3 complex is involved in the recognition and recruitment of the mRNA and plays a critical role in regulating translation initiation. The mammalian homolog of the murine mammary tumor integration site 6 (INT6), also known as eIF-3e or eIFe, is another name for EIF3E. The INT6 gene is associated with mammary tumors and has been implicated in oncogenesis. The viral integration site protein INT-6 homolog, INT6, is the corresponding protein in viral systems. In summary, EIF3E is an important component of the eukaryotic translation initiation factor 3 complex. It is involved in the regulation of protein synthesis and has been associated with mammary tumors.
Source	Rabbit
Specificity	The total EIF3E levels can be detected using the EIF3E Antibody, which specifically targets the endogenous EIF3E. By rearranging the content, we can say that the EIF3E Antibody is designed to detect the endogenous levels of total EIF3E, providing a reliable method for analysis.
Species Reactivity	Human
Predictive reaction species	Pig;Rabbit;Sheep;Dog;Bovine;Horse;
Antigen	EIF3E
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human EIF3E.
MW	52 kDa
Properties
Concentration	1mg/ml
purification	The SulfoLink™ Coupling Resin was employed for the peptide affinity chromatography purification of the antiserum. This resulted in the production of a highly purified antiserum product.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the integrity of the product, it is recommended to store it at a temperature of -20°C for up to one year. It is important to avoid exposing it to repeated freeze and thaw cycles, as this may compromise its quality and effectiveness. It is always best to follow the recommended storage instructions to ensure that the product remains in optimal condition for use.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773). Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway (PubMed:17468741). May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins (PubMed:17310990, PubMed:17324924).
Tissue specificity	Ubiquitously expressed. Expressed at highest levels in appendix, lymph, pancreas, skeletal muscle, spleen and thymus.
Celluar localization	Cytosol;Extracellular region or secreted;Nucleus;
UniPort	P60228