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Rabbit Anti-EIF3B Polyclonal Antibody#abs137906

Please note that the price provided here is only for your reference. For detailed pricing information, you can contact our seller, Vecent. Western blot analysis EIF3B using COS7 whole cell lysates This product is for research use only, not for use in diagnostic prodecures or in human.

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Description

Catalog-specification	Delivery time	USD price
abs137906-100ug	1-2 Weeks	301.0
abs137906-50ug	1-2 Weeks	201.0

Please note that the price provided here is only for your reference. For detailed pricing information, you can contact our seller, Vecent.

Overview
catalog	abs137906
Other names	Some rearranged content based on the original text information: 1. Eukaryotic translation initiation factor 3 subunit B (EIF3B_HUMAN) is also known as eIF-3-eta or eIF3 eta. 2. Another variant of this subunit is called eIF3 p110 or EIF3-P110. 3. There is also a form known as eIF3 p116 or EIF3-P116. 4. EIF3-ETA or EIF3S9 (formerly EIF3S9) is the eta isoform of eukaryotic translation initiation factor 3 subunit 9. 5. This subunit is sometimes referred to as eIF3b. 6. It is associated with the human gene EIF3S9 and is responsible for protein synthesis. 7. In Saccharomyces cerevisiae, it is homologous to a gene called Protein synthesis defective at 36 degrees celsius 1 (hPrt1). 8. The Prt1 homolog is involved in maintaining proper translation initiation in yeast.
Source	Rabbit
Specificity	The detection of endogenous levels of total EIF3B can be achieved using the EIF3B antibody. To generate highly similar content, the following rearrangement can be used: The EIF3B antibody is capable of detecting total EIF3B at its endogenous levels.
Species Reactivity	Human;Mouse
Antigen	EIF3B
Application	The recommended dilution for Western blotting (WB) is 1:1000-3000, for immunohistochemistry (IHC) it's 1:200, and for ELISA using a peptide it's 1:20000-1:40000. It is important to note that the dilution ratios may vary depending on the specific experiment and the concentration of your target protein or peptide. Therefore, it is advisable to optimize the dilution ratio for your specific experimental conditions to achieve optimal results.
Immunogen	A synthesized peptide derived from human EIF3B.
MW	116 kDa
Properties
Concentration	1mg/ml
purification	Through the use of SulfoLink™ Coupling Resin, peptide affinity chromatography was performed to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the integrity of the product, it is recommended to keep it refrigerated at a temperature of -20 °C for a period of one year. It is important to avoid subjecting it to repeated cycles of freezing and thawing, as this may compromise its efficacy. It is advisable to handle this product with care and to follow the storage instructions closely to ensure its optimal performance.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:9388245, PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:9388245, PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).
Posttranslational modification	Phosphorylated. Phosphorylation is enhanced upon serum stimulation.
Celluar localization	Cytosol;Extracellular region or secreted;
UniPort	P55884