Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-CYP2E1 Polyclonal Antibody#abs136403

Description

Catalog-specification	Delivery time	USD price
abs136403-100ug	1-2 Weeks	301.0
abs136403-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is for your reference only. For detailed pricing, kindly get in touch with our seller Vecent. It is important to understand that the mentioned price is subject to change as per the prevailing market conditions. Therefore, we would recommend you to reach out to our seller and get a better understanding of the product pricing and any other related queries. Vecent would be happy to assist you with all the necessary information and help you make an informed decision.

Overview
catalog	abs136403
Description	This particular gene falls under the cytochrome P450 superfamily, which is known for producing monooxygenase enzymes responsible for various reactions associated with drug metabolism. Apart from this, the gene also synthesizes cholesterol, steroids, and other lipids. It localizes to the endoplasmic reticulum and is usually activated by ethanol and a diabetic state, as well as starvation. This enzyme can metabolize both endogenous and exogenous substrates such as benzene, carbon tetrachloride, and nitrosamines found in cigarette smoke. The number of substrates catalyzed by the enzyme implies that it plays a role in different processes such as cancer, diabetes, hepatic cirrhosis, and gluconeogenesis. This was an observation gathered from RefSeq in July 2008.
Other names	Ethanol-inducible P450, also known as cytochrome P450 2E1 or CYP2E1, is an enzyme responsible for the metabolism of various substances in the body. It belongs to the cytochrome P450 family, specifically the subfamily II E. This enzyme plays a crucial role in the oxidation of alcohol and other compounds, making it an essential component of the body's detoxification system. The gene encoding for ethanol-inducible P450 is located in humans, and it is expressed predominantly in the liver. This enzyme is known for its ability to metabolize a wide range of toxic compounds, including drugs, carcinogens, and environmental pollutants. It uses oxygen and NADPH as cofactors to catalyze the hydroxylation of its substrate molecules. Ethanol-inducible P450 has been linked to the metabolism of several drugs, including acetaminophen, benzene, and ethanol. It is also involved in the activation of procarcinogens, which can lead to the formation of DNA adducts and ultimately contribute to the development of cancer. In addition to its role in drug metabolism and detoxification, ethanol-inducible P450 is also implicated in the bioactivation of several compounds, leading to toxic effects. It has been suggested that the overexpression of this enzyme may contribute to the development of liver diseases, including alcohol-induced liver damage and hepatocellular carcinoma. Overall, ethanol-inducible P450 is a critical enzyme involved in the metabolism and detoxification of various substances in the body. Its activity and expression can be influenced by factors such as genetic variations, environmental exposures, and lifestyle choices. Further research is needed to fully understand the role of this enzyme in human health and disease.
Source	Rabbit
Specificity	The CYP2E1 Antibody is capable of detecting the total amount of endogenous CYP2E1 present in the sample. This antibody recognizes all forms of CYP2E1 expressed in the sample and allows for accurate detection of its levels.
Species Reactivity	Human;Mouse;Rat
Antigen	CYP2E1
Application	ELISA(peptide) 1:20000-1:40000, IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:500-1:2000. Rearranging the original content, the new content is: The recommended dilution ranges for different techniques are as follows: For ELISA (peptide), the dilution range is 1:20000-1:40000. For immunohistochemistry (IHC), the dilution range is 1:50-1:200. For immunofluorescence/immunocytochemistry (IF/ICC), the dilution range is 1:100-1:500. And for Western blotting (WB), the dilution range is 1:500-1:2000.
Immunogen	A synthesized peptide derived from human CYP2E1.
MW	57kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms.
Tissue specificity	By ethanol and isoniazid.
Celluar localization	Endoplasmic reticulum;Golgi apparatus;Mitochondrion;
UniPort	P05181