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Rabbit Anti-Collagen XVII Polyclonal Antibody#abs133252

Please note that the price mentioned above is provided as a reference only. For detailed pricing information, we kindly ask you to contact our seller, Vecent. Western blot analysis Collagen XVII using HepG2 whole cell lysates This product is for research use only, not for use in diagnostic...

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Description

Catalog-specification	Delivery time	USD price
abs133252-100ug	1-2 Weeks	301.0
abs133252-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is provided as a reference only. For detailed pricing information, we kindly ask you to contact our seller, Vecent.

Overview
catalog	abs133252
Other names	The protein known as bullous pemphigoid antigen 2 has a molecular weight of 180 kDa. It is also referred to as alpha 1 type XVII collagen, BA16H23.2, BP 180, BP180, BPA 2, BPAG 2, BPAG2, bullous pemphigoid antigen 2, COL17A1, collagen 17, collagen alpha 1 XVII chain, collagen alpha 1(XVII) chain, collagen alpha1 XVII chain, collagen type XVII alpha 1, collagen XVII alpha 1 polypeptide, CollagenXVII, epidermolysis bullosa junctional localisata variant, FLJ60881, KIAA0204, LAD 1, and LAD1. This protein plays a significant role in diseases such as bullous pemphigoid and epidermolysis bullosa junctional localisata variant. It is also known for its involvement in collagen synthesis and functioning as an adhesion molecule in the skin. Its genetic code is represented by the COL17A1 gene.
Source	Rabbit
Specificity	The detection of endogenous levels of total Collagen XVII is accomplished using the Collagen XVII Antibody. It is important to note that the content generated by rearranging the original text may vary significantly from the provided information.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Dog;
Antigen	Collagen XVII
Application	For western blotting, a dilution range of 1:1000-3000 is recommended, while for immunohistochemistry, a dilution of 1:200 is suggested. For ICC/IF, as well as ELISA using peptide, a dilution range of 1:20000-1:40000 can be used. It is important to note that these dilution ranges may vary depending on the specific experiment and conditions being used.
Immunogen	XVII。，。
MW	130,180kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized to purify the antiserum through the process of peptide affinity chromatography. The resulting product was of high quality, meeting the required standards for use in further research and experimentation.
Clonality	Polyclonal Antibody
Stability & Storage	Ensure that the product is stored at a temperature of -20 °C for a period of one year without experiencing repeated freeze/thaw cycles. It is important to avoid subjecting the product to fluctuations in temperature as this may affect its quality and stability. Taking proper precautions during storage will help maintain the integrity of the product over time.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	May play a role in the integrity of hemidesmosome and the attachment of basal keratinocytes to the underlying basement membrane.
Tissue specificity	Detected in skin (PubMed:8618013). In the cornea, it is detected in the epithelial basement membrane, the epithelial cells, and at a lower level in stromal cells (at protein level) (PubMed:25676728). Stratified squamous epithelia. Found in hemidesmosomes. Expressed in cornea, oral mucosa, esophagus, intestine, kidney collecting ducts, ureter, bladder, urethra and thymus but is absent in lung, blood vessels, skeletal muscle and nerves.
Posttranslational modification	The intracellular/endo domain is disulfide-linked.Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.The ectodomain is shedded from the surface of keratinocytes resulting in a 120-kDa soluble form, also named as 120 kDa linear IgA disease antigen. The shedding is mediated by membrane-bound metalloproteases. This cleavage is inhibited by phosphorylation at Ser-544.
Celluar localization	Endoplasmic reticulum;Extracellular region or secreted;Plasma Membrane;
UniPort	Q9UMD9