Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-CD68 Polyclonal Antibody#abs137004

Please note that the price mentioned above is only for your reference. For detailed pricing information, kindly get in touch with our seller, Vecent. Western blot analysis CD68 using HT-29 whole cell lysates This product is for research use only, not for use in diagnostic prodecures or in human.

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs137004-100ug	1-2 Weeks	301.0
abs137004-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is only for your reference. For detailed pricing information, kindly get in touch with our seller, Vecent.

Overview
catalog	abs137004
Other names	CD68, also known as CD68 antigen or CD68 molecule, is an important protein that is found on the surface of macrophages. It is also referred to as microsialin or macrosialin. The gene that codes for CD68 is known as SCARD1 or scavenger receptor class D member 1. Other common names for CD68 include gp11 and Gp110. CD68 is a vital marker used in various diagnostic and research applications related to macrophages. It is predominantly expressed in monocytes and macrophages but can also be found in other cell types such as dendritic cells and neutrophils. CD68 has been found to play a crucial role in various cellular processes, including phagocytosis, antigen presentation, and lysosomal biogenesis. The CD68 protein has a molecular weight of approximately 110 kDa, and it is primarily localized in the lysosomal compartment of the cells. It has been suggested that CD68 may function as a scavenger receptor, allowing the macrophages to clear up debris or damaged cells in the body. Additionally, CD68 has also been linked to various diseases such as atherosclerosis, Alzheimer's disease, and cancer. Overall, CD68 is an essential protein that plays a critical role in the immune response of the body. Its expression and function provide a valuable tool for the diagnosis and treatment of various diseases.
Source	Rabbit
Specificity	The CD68 Antibody is capable of identifying the total CD68 protein at its natural levels within the body. This information serves as the basis for generating highly similar content which rearranges the previous sentence in order to convey the same message.
Species Reactivity	Human;Mouse;Rat
Antigen	CD68
Application	For Western blotting, a dilution of 1:1000 to 1:3000 can be used, while for immunohistochemistry, a dilution of 1:50 to 1:200 is recommended. For immunofluorescence and immunocytochemistry, a dilution range of 1:100-1:500 is suitable. When performing ELISA assays using peptide antigens, a dilution range of 1:20000 to 1:40000 is recommended. It is important to note that appropriate dilution factors may vary depending on the specific antibodies and experimental conditions being used.
Immunogen	A synthesized peptide derived from human CD68.
MW	36 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent purification by means of peptide affinity chromatography. The resulting product was highly refined and optimized for its intended use.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the integrity of the product, we recommend storing it at a temperature of -20 °C for up to one year. It is important to avoid any kind of repeated freezing and thawing cycles, as this could compromise the quality of the product. Therefore, it is recommended to only thaw the product when necessary and store it at the recommended temperature at all times to ensure optimal performance.
Storage buffer	This is a revised version of the original text: To ensure stability and maintain the quality of Rabbit IgG, it is recommended to store it at -20 °C in a solution containing phosphate buffered saline (pH 7.4), 150mM NaCl, 0.02% sodium azide, and 50% glycerol. This formulation provides an optimal environment for preserving the integrity and functionality of the antibody. It is important to note that the Rabbit IgG is stable for a period of 12 months from the date of receipt. By following these storage guidelines, you can ensure the longevity and effectiveness of the Rabbit IgG for your experiments and research.
Target
Background	Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
Tissue specificity	Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
Posttranslational modification	N- and O-glycosylated.
Celluar localization	Endosome;Lysosome;Plasma Membrane;
UniPort	P34810