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Rabbit Anti-CCL13 Polyclonal Antibody#abs139431

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Description

Catalog-specification	Delivery time	USD price
abs139431-100ug	1-2 Weeks	301.0
abs139431-50ug	1-2 Weeks	201.0

Overview
catalog	abs139431
Other names	C-C motif chemokine 13, also known as CCL13 or CCL13_HUMAN, is a protein involved in immune system regulation. It is classified as a chemokine, specifically belonging to the C-C motif family. Some other names it goes by include Chemokine (C C motif) ligand 13, CK beta 10, CK-beta-10, CKb10, MCP-4, MGC17134, Monocyte chemoattractant protein 4, Monocyte chemotactic protein 4, NCC 1, NCC-1, NCC1, New CC chemokine 1, SCYA13, SCYL1, short chain, Small inducible cytokine A13 precursor, and Small inducible cytokine subfamily A (Cys Cys) member 13. This protein plays a role in the recruitment and activation of monocytes, which are a type of white blood cell important in immune response. Its function is mediated through binding to specific receptors on cell surfaces. Understanding the role of CCL13 in immune system regulation can provide insights for the development of therapeutic interventions targeting immune-related disorders.
Source	Rabbit
Specificity	The detection of endogenous levels of total CCL13 is possible using the CCL13 Antibody. By rearranging the content, it can be stated that the CCL13 Antibody is designed to specifically detect the total levels of endogenous CCL13 present in a sample.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Dog
Antigen	CCL13
Application	The recommended dilution ratios for Western Blot (WB) range from 1:1000 to 1:3000, while for Immunohistochemistry (IHC) it is recommended to use a dilution of 1:200. For ELISA using peptide, a dilution range of 1:20000 to 1:40000 is suggested. It is important to follow these dilution guidelines to ensure accurate and reliable results. Properly diluting the samples allows for optimal detection and avoids non-specific binding.
Immunogen	A synthesized peptide derived from human CCL13.
MW	11 kDa
Properties
Concentration	1mg/ml
purification	The use of SulfoLink™ Coupling Resin in peptide affinity chromatography allowed for the purification of the antiserum. The resulting purified antiserum is highly effective and reliable, making it an ideal choice for a wide range of applications. This process is a crucial step in producing high-quality antiserum that can be used in a variety of research and medical applications. Overall, peptide affinity chromatography is a powerful tool for isolating specific proteins and other molecules with high levels of purity, and it has many potential uses in the fields of medicine, biotechnology, and research.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure maximum effectiveness, it is recommended that the product be stored at a temperature of -20 °C for a period of one year. It is important to note that repeated freeze/thaw cycles should be avoided as this can potentially compromise the integrity of the product.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens.
Tissue specificity	Widely expressed. Found in small intestine, thymus, colon, lung, trachea, stomach and lymph node. Low levels seen in the pulmonary artery smooth muscle cells.
Posttranslational modification	One major form (form long), and two minor forms (short chain and medium chain) are produced by differential signal peptide cleavage. The medium chain is about 30-fold less active than the long chain.
Celluar localization	Extracellular region or secreted;
UniPort	Q99616