Rabbit Anti-PI3 Kinase P110alpha Polyclonal Antibody#abs131188

abs131188-50ug

1-2 Weeks

201

abs131188-100ug

1-2 Weeks

301

Description

Phosphoinositide-3-kinase (PI3K) is an enzyme that phosphorylates different types of phosphatidylinositols - PtdIns, PtdIns4P and PtdIns(4,5)P2 - to produce phosphatidylinositol 3,4,5-trisphosphate (PIP3). This molecule has a crucial role in recruiting proteins that contain PH (pleckstrin homology) domains to the cell membrane, such as AKT1 and PDPK1. These proteins then activate signaling pathways that control various cellular processes, such as growth, survival, proliferation, motility and morphology. The activation of PI3K signaling is usually triggered by the interaction of growth factors with specific receptors on the cell surface. In this way, PI3K plays an essential role in mediating cellular responses to changes in the extracellular environment.

Other names

The 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha, also known as caPI3K, CLOVE, CWS5, MCAP, MCM, MCMTC, p110 alpha, or Phosphatidylinositol-4, is an essential component of the phosphoinositide 3-kinase (PI3K) pathway. This alpha isoform of the catalytic subunit alpha plays a crucial role in regulating cell growth, proliferation, migration, and survival.
The Phosphoinositide 3 kinase catalytic alpha polypeptide is encoded by the gene PIK3CA, which is also known as PI3KC A or Pik3ca. It belongs to the PI3K family that phosphorylates phosphatidylinositol 4,5 bisphosphate to produce phosphatidylinositol 3,4,5 trisphosphate (PIP3). The PIP3 activates downstream signaling molecules such as AKT and mTOR, which initiate several cellular processes.
Mutations in the PIK3CA gene are associated with various human cancers, including breast, ovarian, and colon cancers. These mutations result in the constitutive activation of the PI3K pathway and contribute to tumor formation, growth, and metastasis. Therefore, targeting PI3K activity has emerged as a promising treatment strategy for cancer therapy.
In conclusion, the PI3K catalytic subunit alpha isoform is a critical regulator of cellular processes and its dysregulation leads to the development of cancer. Understanding the role of PI3K in cancer biology holds great promise for targeted therapies in the future.

Source

Rabbit

Specificity

The endogenous levels of total PI3 kinase P110α can be detected using the PI3 kinase P110α Antibody.

Predictive reaction species

Pig;Rabbit;Sheep;Dog;Bovine;Horse;

Application

To achieve optimal results in your experiments, here are the recommended dilutions for this antibody: Western blotting at 1:500-1:2000, immunohistochemistry at 1:50-1:200, immunofluorescence or immunocytochemistry at 1:100-1:500, and ELISA using peptide at 1:20000-1:40000. Following these suggested dilutions will ensure that you obtain reliable and reproducible results with this antibody.

A newly synthesized peptide has been developed from the protein sequence of human PI3 kinase P110α. The peptide is designed to mimic the function of the original protein and has a high degree of similarity to the natural protein structure. This synthesized peptide can be used as a potential therapeutic agent for treating diseases associated with PI3 kinase pathways. The development of this peptide is a significant step forward in the understanding and manipulation of protein function in human biology. Further research is needed to investigate the full potential and applications of this synthesized peptide in the field of medicine.

Properties

1mg/ml

Purification

SulfoLink™ Coupling Resin was utilized in the purification process of the antiserum via peptide affinity chromatography. The resulting product is highly pure and effective in its intended purpose.

Clonality

Polyclonal Antibody

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles

Storage buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Target

Background

 Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. Also has serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS. Plays a role in the positive regulation of phagocytosis and pinocytosis (By similarity).

Celluar localization

P42336