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Rabbit Anti-CBLN1 Polyclonal Antibody#abs141002

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Description

Catalog-specification	Delivery time	USD price
abs141002-100ug	1-2 Weeks	301.0
abs141002-50ug	1-2 Weeks	201.0

Overview
catalog	abs141002
Other names	The protein [des-Ser1]-cerebellin, also known as CBLN1 or cerebellin 1, is a precursor to the cerebellin 1 peptide. Found in humans, this protein plays a role in the function and development of the cerebellum, a part of the brain responsible for motor control and coordination. Precerebellin, a form of the protein before it is cleaved into the active peptide form, is also present. Understanding the function of cerebellin 1 and its precursor may provide insights into neurological disorders related to cerebellar dysfunction. CBLN1 is highly similar to other members of the cerebellin protein family, including CBLN2, CBLN3, and CBLN4.
Source	Rabbit
Specificity	The CBLN1 Antibody is capable of detecting total levels of endogenous CBLN1. To create new content that is highly similar, the original text information should be rearranged.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Chicken;Rabbit;Sheep;Dog;Bovine;Horse;
Antigen	CBLN1
Application	For Western blot analysis, we suggest diluting the primary antibody at 1:500 to 1:1000. For immunofluorescence or immunocytochemistry, a dilution of 1:100 to 1:500 is recommended. For ELISA using a peptide, we recommend a dilution of 1:20000 to 1:40000. It is important to note that these dilutions may need to be optimized for individual experiments and should be tested beforehand.
Immunogen	A synthesized peptide.
MW	25 KD
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was employed in the purification of the antiserum through peptide affinity chromatography. Let's generate a highly similar content by reordering the existing information.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain its quality, the product should be stored at a temperature of -20 °C for a duration of one year. It is crucial to prevent it from undergoing frequent freeze/thaw cycles.
Storage buffer	This Rabbit IgG comes in a solution of phosphate buffered saline at pH 7.4 with 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is best stored at a temperature of -20°C, and will remain stable for up to 12 months from the date of receipt. To ensure its effectiveness, it's important to follow the storage guidelines and use within the designated time frame.
Target
Background	Required for synapse integrity and synaptic plasticity. During cerebellar synapse formation, essential for the matching and maintenance of pre- and post-synaptic elements at parallel fiber-Purkinje cell synapses, the establishment of the proper pattern of climbing fiber-Purkinje cell innervation, and induction of long-term depression at parallel fiber-Purkinje cell synapses. Plays a role as a synaptic organizer that acts bidirectionally on both pre- and post-synaptic components. On the one hand induces accumulation of synaptic vesicles in the pre-synaptic part by binding with NRXN1 and in other hand induces clustering of GRID2 and its associated proteins at the post-synaptic site through association of GRID2. NRXN1-CBLN1-GRID2 complex directly induces parallel fiber protrusions that encapsulate spines of Purkinje cells leading to accumulation of GRID2 and synaptic vesicles. Required for CBLN3 export from the endoplasmic reticulum and secretion (By similarity). NRXN1-CBLN1-GRID2 complex mediates the D-Serine-dependent long term depression signals and AMPA receptor endocytosis (PubMed:27418511).
Tissue specificity	In the Purkinje cells postsynaptic structures. In the cerebellum, cerebellin is much less abundant than [des-Ser1]-cerebellin.
Posttranslational modification	The proteolytic processing to yield cerebellin seems to occur either prior to the secretion by presynaptic neurons and subsequent oligomerization or in some other location after release of the mature protein.
Celluar localization	Extracellular region or secreted;Plasma Membrane;
UniPort	P23435