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Rabbit Anti-CASP1 Polyclonal Antibody#abs135668

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Description

Catalog-specification	Delivery time	USD price
abs135668-100ug	1-2 Weeks	301.0
abs135668-50ug	1-2 Weeks	201.0

Overview
catalog	abs135668
Description	Interleukin-1 converting enzyme, also known as caspase-1, is a type I cysteine protease enzyme. This class of enzymes also includes caspases -4, -5, -11, and -12. Caspase-1 is responsible for cleaving inflammatory cytokines such as pro-IL-1 and interferon-γ inducing factor (IL-18) into their mature forms. The process involves proteolytic activation of the proenzyme, leading to the formation of a tetramer of active subunits p20 and p10. Caspase-1 has a large pro-domain containing a caspase recruitment domain (CARD). Overexpression of caspase-1 can cause apoptosis. Deficiency of caspase-1 in mice does not affect apoptosis but does impair the maturation of pro-IL-1β and renders the animals resistant to endotoxic shock. There are at least six identified isoforms of caspase-1, including caspase-1 α, β, γ, δ, ε and ζ. Most isoforms induce apoptosis upon over-expression, with products ranging between 30-48 kDa. The ε isoform of caspase-1 only contains the p10 subunit and acts as a dominant negative without inducing apoptosis. The ζ isoform lacks 39 amino-terminal residues found in the α isoform but induces apoptosis. Activation of caspase-1 involves the formation of an inflammasome molecular platform that includes caspase-5, Pycard/Asc, and NALP1. This oligomerization process results in the activation of caspase-1 for its cytokine cleavage function.
Other names	CASP-1, also known as Caspase 1 or Interleukin-1 beta convertase, is a vital protein responsible for the activation of Interleukin-1 beta, a cytokine involved in immune and inflammatory responses. CASP-1 has a subunit called p10 or Caspase-1 subunit p10. Other aliases for CASP-1 include ICE, IL-1BC, IL1B convertase, and Interleukin 1B converting enzyme. This protein plays a significant role in programmed cell death or apoptosis, and its dysregulation has been implicated in various diseases, including autoimmune disorders and cancer. Overall, the study of CASP-1 and its function is essential for understanding the pathogenesis of multiple conditions and identifying potential targets for therapeutic interventions.
Source	Rabbit
Specificity	The antibody for CASP1 is capable of identifying the overall levels of endogenous CASP1. To create similar content, the key information should be maintained but presented using different words and sentence structures. Therefore, the CASP1 antibody can be utilized to detect the quantity of CASP1 present in its natural state.
Species Reactivity	Human;Mouse;Rat
Antigen	CASP1
Application	The recommended dilutions for Western blot (WB) range from 1:500 to 1:2000. For Immunohistochemistry (IHC), the suggested dilutions are between 1:50 and 1:200. When performing Immunofluorescence (IF) or Immunocytochemistry (ICC), dilute the antibodies at a ratio of 1:100 to 1:500. For ELISA using peptides, the appropriate dilutions are 1:20000 to 1:40000. It is crucial to follow these dilution guidelines to ensure accurate and reliable results.
Immunogen	A synthesized peptide derived from human CASP1.
MW	45kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of MB21D1/cGAS, rendering it inactive (PubMed:28314590).
Tissue specificity	Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
Posttranslational modification	The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
Celluar localization	Cytosol;Extracellular region or secreted;Mitochondrion;
UniPort	P29466