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Rabbit Anti-APOH Polyclonal Antibody#abs135872

Description

Catalog-specification	Delivery time	USD price
abs135872-100ug	1-2 Weeks	301.0
abs135872-50ug	1-2 Weeks	201.0

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Overview
catalog	abs135872
Description	Apolipoprotein H plays a vital role in several essential physiological processes, such as lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. According to studies, APOH might be a necessary cofactor for anionic phospholipid binding by antiphospholipid autoantibodies present in the sera of lupus and primary antiphospholipid syndrome patients. However, this cofactor is not necessary for antiphospholipid autoantibodies associated with infections. This data was last updated in July 2008.
Other names	Apolipoprotein H, also known as activated protein C binding protein or beta-2-glycoprotein I, plays a crucial role in the regulation of blood clotting and lipid metabolism. This protein acts as an inhibitor of activated protein C and a cofactor for anticardiolipin antibodies. It binds to activated protein C, preventing its activity and thus regulating the clotting process. Additionally, apolipoprotein H is involved in transporting lipids and cholesterol in the blood. It is a glycoprotein and is encoded by the APOH gene. Due to its diverse functions, apolipoprotein H is implicated in various physiological and pathological processes, including thrombosis, cardiovascular diseases, and autoimmune disorders. Study of this protein offers insights into the intricate mechanisms underlying clotting and lipid regulation in the human body.
Source	Rabbit
Specificity	The APOH Antibody has the ability to recognize and bind to total endogenous levels of APOH. This means that the antibody can detect the entire amount of APOH present in a sample. By using the APOH Antibody in experiments, researchers can accurately measure the levels of this important protein and gain a better understanding of its role in biological processes.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Sheep;Bovine;Dog;Horse;
Antigen	APOH
Application	The recommended dilution range for Western blot is 1:500-1:2000, while for Immunohistochemistry it's 1:50-1:200. If you're working with ELISA using peptide, a dilution range of 1:20000-1:40000 is advised. It's important to carefully follow the recommended dilution range for each assay to ensure accurate and reproducible results. By adjusting the dilution ratio, you can optimize the signal-to-noise ratio of your assay, enabling reliable detection of your target molecule. Remember, the proper dilution strategy is a critical component of any successful protein analysis experiment.
Immunogen	A synthesized peptide derived from human APOH.
MW	38kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.
Tissue specificity	Expressed by the liver and secreted in plasma.
Posttranslational modification	N- and O-glycosylated. PubMed:6587378 also reports glycosylation on 'Asn-188' for their allele.
Celluar localization	Extracellular region or secreted;
UniPort	P02749