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Rabbit Anti-APG5L/ATG5 Polyclonal Antibody#abs137059

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Description

Catalog-specification	Delivery time	USD price
abs137059-100ug	1-2 Weeks	301.0
abs137059-50ug	1-2 Weeks	201.0

Overview
catalog	abs137059
Other names	ATG5, also known as APG5 or Autophagy protein 5, is a crucial component involved in the autophagy process. It plays a significant role in maintaining cellular homeostasis by facilitating the degradation and recycling of cellular components. ATG5 is a homolog of the autophagy-related gene 5 in Saccharomyces cerevisiae, indicating its evolutionary conservation and importance across species. ATG5 functions by forming a complex with other autophagy-related proteins, including ATG12 and ATG16L1. This complex participates in the elongation and closure of the isolation membrane, leading to the formation of the autophagosome. The autophagosome then fuses with lysosomes, where its content gets degraded by lysosomal enzymes. The protein has been associated with various biological processes apart from autophagy. It also has a role in apoptosis, the process of programmed cell death. In apoptosis, ATG5 interacts with apoptosis-specific proteins (ASP), promoting cell death. Therefore, it's also referred to as Apoptosis-specific protein. ATG5 is widely expressed in different tissues and is essential for maintaining cellular integrity. Its dysfunction has been implicated in various diseases, including neurodegenerative disorders, cancer, and metabolic disorders. Research and advancements in understanding ATG5 and its functional significance continue to shed light on its potential therapeutic targets and applications in disease treatment.
Source	Rabbit
Specificity	The APG5L/ATG5 Antibody is able to detect the entire spectrum of endogenous APG5L/ATG5 protein levels.
Species Reactivity	Human;Mouse;Rat
Antigen	APG5L/ATG5
Application	Here are alternative ways to express the original information: 1. For Western blotting, dilute the sample in a ratio of 1:1000-3000. In order to detect the protein of interest, immunohistochemistry requires a dilution of 1:200. For ELISA using a peptide, you will need to dilute the sample between 1:20000-1:40000. 2. If you're planning on doing Western blotting, make sure to dilute your sample at a ratio of 1:1000-3000. As for immunohistochemistry, you'll need a dilution of 1:200 to detect the protein. For ELISA with a peptide, the recommended dilution is between 1:20000-1:40000. 3. To perform Western blotting, the sample should be diluted at a ratio of 1:1000-3000. If you're going to do immunohistochemistry, you'll need to dilute your sample 1:200 to detect the protein. ELISA using a peptide requires a dilution between 1:20000-1:40000. 4. Dilute the sample at a ratio of 1:1000-3000 for Western blotting. Immunohistochemistry requires a dilution of 1:200 to detect the protein. ELISA using a peptide requires a dilution between 1:20000-1:40000. 5. The sample should be diluted at a ratio of 1:1000-3000 for Western blotting. If you're doing immunohistochemistry, you'll need to dilute your sample 1:200 to detect the protein. ELISA using a peptide requires a dilution between 1:20000-1:40000 in order to achieve accurate results.
Immunogen	A human APG5L/ATG5 derived peptide has been synthesized, and a highly similar content can be generated by using the original text information to rearrange the words and phrases. Offering no resemblance to the way ChapGPT generates content, this method utilizes a language model to produce unique text.
MW	55 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. To achieve this, a process was implemented to isolate the specific peptides required and extract them from the mixture. Through this process, a highly purified antiserum was obtained, ready to be used for various applications.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure the preservation of the product, it is recommended to store it at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles.
Storage buffer	This is a description of Rabbit IgG, which is suspended in a solution of phosphate buffered saline at pH 7.4, with 150 mM NaCl and 0.02% sodium azide. It contains 50% glycerol and should be stored at a temperature of -20 °C. This product has a stable shelf life of 12 months from the date of receipt.
Target
Background	Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. The ATG12-ATG5 conjugate also negatively regulates the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Also plays a role in translation or delivery of incoming viral RNA to the translation apparatus. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.
Tissue specificity	Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.
Posttranslational modification	Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.Acetylated by EP300.
Celluar localization	Cytoskeleton;Cytosol;
UniPort	Q9H1Y0