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Rabbit Anti-AHCY Polyclonal Antibody#abs136772

Just a quick note that the price mentioned here is for your reference only. To get detailed pricing information, please get in touch with our seller Vecent. Western blot analysis of extracts of various celllines, using RAD51 antibody. This product is for research use only, not for use in...

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Description

Catalog-specification	Delivery time	USD price
abs136772-100ug	1-2 Weeks	301.0
abs136772-50ug	1-2 Weeks	201.0

Just a quick note that the price mentioned here is for your reference only. To get detailed pricing information, please get in touch with our seller Vecent.

Overview
catalog	abs136772
Description	The adenosylhomocysteinase family includes S-adenosylhomocysteine hydrolase, which plays a crucial role in regulating transmethylation reactions. It belongs to this family as it catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) into adenosine (Ado) and L-homocysteine (Hcy). This enzyme is responsible for maintaining the intracellular S-adenosylhomocysteine (SAH) concentration at the appropriate level. Deficiency in S-adenosylhomocysteine hydrolase can result in hypermethioninemia, a condition characterized by elevated levels of methionine. Interestingly, there are different isoforms of this enzyme, which are produced through alternative splicing of its transcript variants. These isoforms contribute to the diverse functions and regulation of S-adenosylhomocysteine metabolism in cells.
Other names	SAHH, also known as adenosylhomocysteinase or AdoHcyase, is an essential enzyme involved in the hydrolysis of S-adenosyl-L-homocysteine (AdoHcy) into adenosine and L-homocysteine. It plays a crucial role in maintaining a balance between AdoHcy and S-adenosylmethionine (AdoMet), which are key metabolites in methylation reactions. The protein encoded by the SAHH gene, also called ahcY, is responsible for the catalytic activity of SAHH. It is found in various tissues and cells throughout the body, including the liver, kidney, brain, and immune cells. SAHH is highly conserved across species and is critical for normal cellular function. Deficiencies or mutations in SAHH can lead to hyperhomocysteinemia, a condition characterized by elevated levels of homocysteine in the blood. This condition is associated with an increased risk of cardiovascular diseases, neurological disorders, and developmental abnormalities. Understanding the structure and function of SAHH has important implications for the development of therapeutic strategies targeting methylation-related diseases. Future research in this field may provide insights into the regulation of SAHH activity and its potential as a therapeutic target. Overall, SAHH plays a crucial role in maintaining cellular homeostasis and is a key enzyme in methylation reactions.
Source	Rabbit
Specificity	Detection of endogenous levels of total AHCY is possible with the AHCY Antibody. The antibody is designed to bind specifically to AHCY, allowing for the accurate measurement of total AHCY expression. This capability is crucial for studying the role and regulation of AHCY in various biological processes. Researchers can utilize the AHCY Antibody to investigate the impact of AHCY on cellular functions and its potential involvement in disease development. By detecting endogenous AHCY levels, valuable insights can be gained into the physiological relevance of this enzyme.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Zebrafish;Chicken;Rabbit;Xenopus;Dog;Horse;Bovine;Sheep;Pig
Antigen	AHCY
Application	The recommended dilution for Western blotting ranges from 1:500 to 1:2000, while for immunohistochemistry it ranges from 1:50 to 1:200. When performing ELISA with peptides, the dilution factor is recommended to be between 1:20000 and 1:40000. It is important to follow these guidelines to obtain accurate and reliable results in your experiments.
Immunogen	A synthesized peptide derived from human AHCY.
MW	48kDa
Properties
Concentration	1mg/ml
purification	To obtain a purified antiserum, a peptide affinity chromatography technique was employed, which involved coupling the SulfoLink™ Coupling Resin with specific peptides that could bind to the desired antibodies. This purification process helped isolate the targeted antiserum while removing other undesirable substances.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.
Celluar localization	Cytosol;Extracellular region or secreted;Nucleus;
UniPort	P23526