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Rabbit Anti-ACADM Polyclonal Antibody#abs136190

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Description

Catalog-specification	Delivery time	USD price
abs136190-100ug	1-2 Weeks	301.0
abs136190-50ug	1-2 Weeks	201.0

Overview
catalog	abs136190
Description	Medium-chain acyl-CoA dehydrogenase (MCAD) is an essential enzyme involved in the first step of mitochondrial fatty acid beta-oxidation. Encoded by a specific gene, MCAD functions as a homotetramer and specifically acts on straight chain fatty acids ranging from C4 to C12. Defects in this gene lead to a metabolic disorder known as medium-chain acyl-CoA dehydrogenase deficiency. This condition is characterized by liver dysfunction, episodes of low blood sugar during fasting, and encephalopathy. In severe cases, it can even lead to fatal outcomes, particularly in infants. Variants of the gene, resulting from alternative splicing, have been identified, giving rise to different isoforms of MCAD.
Other names	The ACAD1 gene codes for the enzyme Acyl coenzyme A dehydrogenase (MCADH), which is responsible for the breakdown of medium chain fatty acids in mitochondria. This oxidation is vital for energy production and the MCAD enzyme plays a crucial role in the fatty acid metabolism pathway. Variations in the ACAD1 gene can result in deficiencies of MCAD, which can lead to serious health issues such as hypoglycemia and metabolic acidosis. Some common alternative names for the ACAD1 gene include Acyl coenzyme A dehydrogenase C 4 to C 12 straight chain, Medium chain specific acyl CoA dehydrogenase mitochondrial, and Medium-chain specific acyl-CoA dehydrogenase. Understanding the function and variations of this gene can help in the diagnosis and treatment of related metabolic disorders.
Source	Rabbit
Specificity	The ACADM Antibody is capable of recognizing total ACADM levels present in the body. Its detection ability is applied to endogenous quantities of ACADM. To create similar content, the original text's information can be reorganized while maintaining its essence.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Bovine;Rabbit;
Antigen	ACADM
Application	The dilutions for Western Blot (WB) range from 1:500 to 1:2000, for Immunohistochemistry (IHC) they range from 1:50 to 1:200, and for ELISA (peptide) the dilutions range from 1:20000 to 1:40000. Please note that the generated content may differ significantly from the original text.
Immunogen	A synthesized peptide derived from human ACADM.
MW	46kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Acyl-CoA dehydrogenase specific for acyl chain lengths of 4 to 16 that catalyzes the initial step of fatty acid beta-oxidation. Utilizes the electron transfer flavoprotein (ETF) as an electron acceptor to transfer electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
Posttranslational modification	Acetylation at Lys-307 and Lys-311 in proximity of the cofactor-binding sites reduces catalytic activity (By similarity). These sites are deacetylated by SIRT3.
Celluar localization	Extracellular region or secreted;Mitochondrion;Nucleus;
UniPort	P11310