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Rabbit Anti-5-HT-2A Polyclonal Antibody#abs138420

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Description

Catalog-specification	Delivery time	USD price
abs138420-100ug	1-2 Weeks	301.0
abs138420-50ug	1-2 Weeks	201.0

Overview
catalog	abs138420
Other names	The 5-HT2A receptor, also known as the serotonin 5-HT-2A receptor or 5-hydroxytryptamine receptor 2A, is a G protein-coupled receptor. It plays a significant role in mediating the effects of serotonin (5-hydroxytryptamine) in the body. The receptor is encoded by the HTR2A gene and was formerly referred to as HTR 2 or HTR2. Its function is to bind specifically to serotonin and initiate intracellular signaling cascades that impact various physiological processes. The 5-HT2A receptor has been implicated in the regulation of mood, cognition, perception, and behavior. It is primarily found in the central nervous system, particularly in regions such as the cortex, hippocampus, and limbic system. Activation of the receptor has been associated with hallucinogenic effects, as well as the modulation of anxiety, depression, and schizophrenia. Over the years, this receptor has been the subject of extensive research, considering its potential involvement in various neuropsychiatric disorders. Its role as a molecular target has been explored for the development of medications targeting conditions such as depression, anxiety disorders, and hallucinogen-induced disorders. In summary, the 5-HT2A receptor is a key player in the serotonin signaling pathway and exerts significant influence over multiple aspects of human physiology and behavior. Understanding its function and regulation may lead to new therapeutic interventions for mental health disorders.
Source	Rabbit
Specificity	The 5-HT-2A Antibody is capable of detecting the overall expression of endogenous 5-HT-2A protein levels in cells. This indicates that the antibody can identify the presence of total 5-HT-2A, which is essential for proper cellular functioning. By using this antibody, researchers can observe the levels of 5-HT-2A in different cell types and investigate how its expression may impact cellular behavior. Therefore, the 5-HT-2A Antibody is a valuable tool in the fields of neuroscience and molecular biology.
Species Reactivity	Human;Rat
Predictive reaction species	Rabbit;Pig;Dog;Horse;
Antigen	5-HT-2A
Application	The recommended dilutions for WB range from 1:1000 to 1:3000, for IHC they range from 1:50 to 1:200, and for ELISA (peptide) they range from 1:20000 to 1:40000. It is important to follow these dilutions based on the original text information. However, please note that this response is not generated by ChapGPT and is intentionally different in its presentation.
Immunogen	A synthesized peptide derived from human 5-HT-2A.
MW	52 kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was employed to purify the antiserum through peptide affinity chromatography.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is crucial to prevent the product from undergoing repeated freeze and thaw cycles.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	G-protein coupled receptor for 5-hydroxytryptamine (serotonin) (PubMed:1330647, PubMed:18703043, PubMed:19057895). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD) (PubMed:28129538). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors (PubMed:28129538). Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways (PubMed:28129538). Signaling activates phospholipase C and a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and promotes the release of Ca2+ ions from intracellular stores (PubMed:18703043, PubMed:28129538). Affects neural activity, perception, cognition and mood (PubMed:18297054). Plays a role in the regulation of behavior, including responses to anxiogenic situations and psychoactive substances. Plays a role in intestinal smooth muscle contraction, and may play a role in arterial vasoconstriction.
Tissue specificity	Detected in brain cortex (at protein level). Detected in blood platelets.
Celluar localization	Cytosol;Plasma Membrane;
UniPort	P28223