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Phospho-ULK1(S556) Rabbit Polyclonal Antibody#abs137067

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Description

Catalog-specification	Delivery time	USD price
abs137067-100ug	1-2 Weeks	301.0
abs137067-50ug	1-2 Weeks	201.0

Overview
catalog	abs137067
Other names	The ATG1 gene, also known as Serine/Threonine Protein Kinase ULK1 or Unc-51 like kinase 1, is a homolog of the Autophagy Related protein 1. It is encoded by the KIAA0722 gene and is responsible for regulating autophagy, a process in which cells break down and digest their own components. ATG1 plays a crucial role in maintaining cellular homeostasis and preventing the accumulation of damaged and toxic molecules. The human ATG1 gene is located on chromosome 12 and consists of 67 exons. It is expressed in various tissues, including the brain, liver, and skeletal muscle. ATG1 has been shown to interact with several proteins involved in autophagy, such as ATG13, FIP200, and ATG101. ATG1 is highly conserved among different species, including humans, mice, and nematodes. In fact, the nematode Caenorhabditis elegans was the first organism in which the ATG1 gene was identified. This led to the discovery of the Unc-51 gene, which was found to be necessary for axon elongation during the development of the nervous system. Overall, ATG1 is a crucial gene that plays a crucial role in autophagy and cellular homeostasis. Its discovery has led to a better understanding of this important process and has provided insights into the development of therapeutics targeting autophagy-related diseases.
Source	Rabbit
Specificity	When ULK1 is phosphorylated at S556, the Phospho-ULK1(S556) Antibody can detect it effectively. This antibody specifically targets endogenous levels of ULK1 and recognizes it only when phosphorylated at S556. To create similar content by rearranging the above information: The Phospho-ULK1(S556) Antibody is capable of detecting ULK1 at its endogenous levels but only when it is phosphorylated at S556. This highly specific antibody ensures accurate detection of phosphorylated ULK1, allowing researchers to study its role and function in various biological processes.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Horse;Sheep
Antigen	ULK1
Application	IHC 1:200, WB 1:1000-3000, and ELISA (peptide) 1:20000-1:40000 are the recommended dilution ratios for different detection methods. These ratios indicate the optimal concentration of antibodies or peptides to use for accurate results. It is crucial to follow these guidelines to ensure reliable data in experiments.
Immunogen	A new peptide has been created which is based on the human Phospho-ULK1(S556). The peptide has been synthesized using advanced techniques to ensure its high similarity to the original protein. This new peptide holds great promise for research in the field of biochemistry and could help to deepen our understanding of cell processes in the human body. The development of this peptide represents a significant breakthrough in the field of peptide synthesis and could potentially lead to further advancements in this area.
MW	120 kDa
Properties
Concentration	1mg/ml
purification	The affinity-purified antibody was obtained from rabbit serum that underwent sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165).
Tissue specificity	Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
Posttranslational modification	Autophosphorylated. Phosphorylated under nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency, phosphorylated by MTOR/mTOR, disrupting the interaction with AMPK and preventing activation of ULK1 (By similarity). In response to nutrient limitation, phosphorylated and activated by AMPK, leading to activate autophagy.
Celluar localization	Cytosol;Endoplasmic reticulum;Endosome;Mitochondrion;
UniPort	O75385