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Phospho-TIF-IA (Ser649) Rabbit Polyclonal Antibody#abs138264

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Description

Catalog-specification	Delivery time	USD price
abs138264-100ug	1-2 Weeks	301.0
abs138264-50ug	1-2 Weeks	201.0

Overview
catalog	abs138264
Other names	The gene AL023001 is also known as RGD1305001, DKFZp566E104, E130302O19Rik, MGC104238, and R75565. Its protein product is called RRN3 or TIF-IA, which stands for Transcription Initiation Factor 1A. RRN3 is a transcription factor that specifically facilitates the initiation of RNA polymerase I-dependent transcription. In other words, it helps kickstart the process that leads to the creation of ribosomal RNA in cells. This function is conserved across species, as yeast also have a protein called RRN3 that performs the same duty.
Source	Rabbit
Specificity	The antibody Phospho-TIF-IA (Ser649) is specifically designed to identify phosphorylated forms of TIF-IA at Ser649, which indicates endogenous levels of TIF-IA. This antibody is unable to detect non-phosphorylated TIF-IA or other forms of TIF-IA phosphorylated at different sites.
Species Reactivity	Human;Mouse;Rat
Antigen	TIF-IA
Application	The recommended dilutions for Western blotting (WB) range from 1:1000 to 1:3000. For immunohistochemistry (IHC), a dilution of 1:200 is suggested. For immunofluorescence/immunocytochemistry (IF/ICC), the optimal dilution range is 1:100 to 1:500. For ELISA using a peptide, the recommended dilution range is 1:20,000 to 1:40,000. It is important to note that the dilutions provided are based on the original text information and should be followed for accurate experimental results.
Immunogen	Phospho-TIF-IA (Ser649) is a fragment of human origin that has been chemically synthesized to create a unique peptide. The synthesis process carefully selects and reorganizes specific amino acids to replicate the original sequence found in human Phospho-TIF-IA (Ser649). This synthesized peptide closely resembles the native protein and retains its functional characteristics. By harnessing the power of chemical engineering, scientists have successfully generated this highly similar peptide for further research and understanding of Phospho-TIF-IA (Ser649) and its physiological functions.
MW	74 kDa
Properties
Concentration	1mg/ml
purification	The purified rabbit serum antibody was obtained through sequential chromatography on affinity columns for phospho- and non-phospho-peptides. The affinity purification method ensured that the antibody was highly purified and specific to its target antigen. This technique is widely used in the production of high-quality antibodies for biomedical research and diagnostic applications. Overall, the affinity purification of the rabbit serum antibody offers a reliable and effective approach to generate highly specific and functional antibodies.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure the preservation of the item, it is recommended to store it consistently at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting it to repeated freeze/thaw cycles, as this can cause damage.
Storage buffer	Phosphate buffered saline containing rabbit IgG is formulated with a pH of 7.4 and comprises 150mM NaCl, 0.02% sodium azide, and 50% glycerol. This product should be stored at a temperature of -20 °C for optimal preservation. It has a shelf life of 12 months starting from the date of receipt.
Target
Background	Required for efficient transcription initiation by RNA polymerase I. Required for the formation of the competent preinitiation complex (PIC). Dissociates from pol I as a consequence of transcription. In vitro, cannot activate transcription in a subsequent transcription reaction (By similarity).
Posttranslational modification	Phosphorylation is required for participation in rDNA transcription (By similarity). Phosphorylated at Thr-200 by MAPK9/JNK2, which abrogates initiation complex formation.
Celluar localization	Nucleus;
UniPort	Q9NYV6