Phospho-PKC Theta (Ser676) Rabbit Polyclonal Antibody#abs131019

abs131019-50ug

1-2 Weeks

201

abs131019-100ug

1-2 Weeks

301

Description

The activation of protein kinase C (PKC), a group of protein kinases that specifically target serine and threonine residues, can occur through the presence of calcium and diacylglycerol, a type of second messenger. One important characteristic of PKC is its ability to phosphorylate a diverse range of protein targets, contributing to various cellular signaling pathways. Additionally, PKC family members are significant receptors for a type of tumor promoter called phorbol esters.

Other names

Protein kinase C theta, also known as nPKC-theta or PRKCT, is a member of the protein kinase C family. It is a type of enzyme that plays a key role in numerous cellular processes, such as cell proliferation, differentiation, and survival. PRKCT is particularly important in immune cells as it is involved in mediating signaling pathways in response to antigen stimulation. This activation leads to the production of cytokines and other mediators that help to mount an effective immune response against infections and other threats. Given its crucial role in immune function, PRKCT has been studied extensively as a potential target for the development of immunomodulatory drugs.

Source

Rabbit

Specificity

The antibody against phospho-PKC theta (Ser676) is designed to specifically recognize and bind to PKC theta protein when it is phosphorylated at Serine 676. This antibody is highly sensitive and capable of detecting endogenous levels of phosphorylated PKC theta.

Predictive reaction species

Pig;Rabbit;Sheep;Dog;Horse;Bovine;

Application

1:500-1:2000，1:50-1:200，/1:100-1:500，（）1:20000-1:40000。，。

One possible rearrangement of the content could be:
The phosphorylation site of Serine 676 in human PKC theta has been used as a basis for synthesizing a peptide. This peptide is a product of chemical synthesis and has been designed to closely resemble the original protein sequence. By targeting this specific region of the protein, researchers hope to gain insights into its functional properties and potential interactions with other cellular molecules. The resulting peptide is expected to be a valuable tool for studying the behavior of PKC theta and its role in various biological processes.

Properties

1mg/ml

Purification

To obtain the antibody, purified rabbit serum was subjected to affinity purification using sequential chromatography on columns specifically designed for phospho- and non-phospho-peptides. This process resulted in a highly pure antibody with strong affinity for its target.

Clonality

Polyclonal Antibody

Please ensure that the product is stored at -20 °C for a period of one year. It is important to avoid subjecting it to repeated freeze/thaw cycles during storage.

Storage buffer

The Rabbit IgG is preserved in a solution of phosphate buffered saline (pH 7.4) containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at a temperature of -20 °C for optimal stability. This product maintains its quality for a period of 12 months from the date of receipt.

Target

Background

This protein is a serine/threonine-protein kinase that plays a crucial role in T-cell receptor (TCR) signaling. It is calcium-independent and relies on phospholipids and diacylglycerol (DAG) for its activation. By activating various transcription factors, such as NF-kappa-B, JUN, NFATC1, and NFATC2, it regulates important processes in T-cell activation, proliferation, differentiation, and survival.
In TCR-CD3/CD28-co-stimulated T-cells, this kinase is essential for the activation of NF-kappa-B and JUN, which are required for the production of interleukin 2 (IL2). It also contributes to the activation of the calcium-dependent NFATC1 and NFATC2, promoting their transactivation. Additionally, it directly phosphorylates CARD11, leading to its association with lipid rafts and recruitment of the BCL10-MALT1 complex. This complex activates the IKK complex and eventually results in the translocation and activation of NF-kappa-B.
Apart from its role in NF-kappa-B signaling, this protein may also indirectly participate in the non-canonical NF-kappa-B pathway. In the pathway leading to JUN activation, it phosphorylates the mediator STK39/SPAK and may not rely on MAP kinases signaling.
During T-cell differentiation, this kinase is crucial for the development of T-helper 2 (Th2) cells and IL17-producing Th17 cells in immune and inflammatory responses. It also plays a minor role in the Th1 response. Moreover, it protects T-cells from apoptosis by phosphorylating BAD and up-regulating BCL-X(L)/BCL2L1 levels through the NF-kappa-B and JUN pathways.
In platelets, this kinase regulates signal transduction downstream of receptors such as ITGA2B, CD36/GP4, F2R/PAR1, and F2RL3/PAR4. It positively influences "outside-in" signaling and granule secretion. Specifically, it may relay signals from the activated ITGA2B receptor by controlling the uncoupling of WASP and WIPF1, thereby modulating actin filament nucleation and branching through the Arp2/3 complex.
Additionally, this kinase may mediate the inhibitory effects of free fatty acids on insulin signaling. It phosphorylates IRS1, blocking its tyrosine phosphorylation and inhibiting downstream activation of the PI3K/AKT pathway. It also phosphorylates MSN in the presence of certain phospholipids and negatively regulates PDPK1's ability to phosphorylate PKB/AKT1 at specific serine residues.
In summary, this calcium-independent, phospholipid- and DAG-dependent serine/threonine-protein kinase plays diverse and crucial roles in TCR signaling, T-cell activation and survival, transcription factor regulation, platelet signaling, and modulation of insulin signaling.

Tissue specificity

Expressed in skeletal muscle, T-cells, megakaryoblastic cells and platelets.

Celluar localization

Cytoskeleton;Cytosol;Plasma Membrane;

Q04759