Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Phospho-MLCK (Tyr464) Rabbit Polyclonal Antibody#abs140216

Note that the price provided is only for your reference. For specific pricing information, please get in touch with our sales representative, Vecent. Rest assured, we will make sure to promptly provide you with all the details you need. Thank you for considering our services. Western blot...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs140216-100ug	1-2 Weeks	301.0
abs140216-50ug	1-2 Weeks	201.0

Overview
catalog	abs140216
Other names	Myosin light chain kinase, also known as MLCK or smooth muscle myosin light chain kinase, is an enzyme that plays a crucial role in smooth muscle contraction. It is encoded by the MYLK gene and exists in different isoforms, including MLCK1, MLCK108, MLCK210, and the smooth muscle isoform (smMLCK). MLCK is involved in the phosphorylation of myosin light chain (MLC), which regulates smooth muscle contraction. It is also essential for cardiac development and maintenance of the blood-brain barrier. In addition to its role in smooth muscle physiology, MLCK has also been implicated in cancer development and progression. It is overexpressed in several types of cancer and is associated with tumor invasion and metastasis. Other names for MLCK include Kinase-related protein (KRP), Telokin, EC 2.7.11.18, MYLK_HUMAN, DKFZp686I10125, MSTP083, FLJ12216, and OTTHUMP00000180642/OTTHUMP00000180643. The protein has a deglutamylated form and has been studied extensively in the context of its kinase activity.
Source	Rabbit
Specificity	The MLCK (Phospho-Tyr464) Antibody is capable of detecting the presence of MLCK only when it is phosphorylated at Tyr464. This antibody is highly selective and specifically targets the phosphorylated form of MLCK without detecting any other non-phosphorylated form. It is designed to accurately detect the endogenous levels of MLCK that are present within the sample. This makes it a highly useful tool for researchers who are seeking to understand the precise role of MLCK in various biological processes, and how it is regulated by phosphorylation. With its high sensitivity and specificity, this antibody provides a reliable means of detecting the phosphorylated form of MLCK in biological samples.
Species Reactivity	Human
Antigen	MLCK
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	"Human MLCK (Phospho-Tyr464) can be utilized to synthesize a novel peptide that closely mimics its structure and function. By leveraging the original information, a highly similar peptide can be designed and produced for various biomedical applications."
MW	211 kDa.
Properties
Concentration	1mg/ml
purification	The purification process involved the use of phospho- and non-phospho-peptide affinity columns to sequentially chromatograph the extracted antibody from rabbit serum. The resulting product was highly purified and derived from the original source.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve its quality, it is advised to store the product at a temperature of -20 °C for a duration of one year. It is important to avoid exposing the product to repeated freeze and thaw cycles. Proper storage conditions will ensure the longevity of the product.
Storage buffer	Store Rabbit IgG in phosphate buffered saline at pH 7.4 with 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be kept at -20 °C and remains stable for a period of 12 months from the date of receipt.
Target
Background	Calcium/calmodulin-dependent myosin light chain kinase implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activity. Phosphorylates PTK2B/PYK2 and myosin light-chains. Involved in the inflammatory response (e.g. apoptosis, vascular permeability, leukocyte diapedesis), cell motility and morphology, airway hyperreactivity and other activities relevant to asthma. Required for tonic airway smooth muscle contraction that is necessary for physiological and asthmatic airway resistance. Necessary for gastrointestinal motility. Implicated in the regulation of endothelial as well as vascular permeability, probably via the regulation of cytoskeletal rearrangements. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis. Plays a role in the regulation of epithelial cell survival. Required for epithelial wound healing, especially during actomyosin ring contraction during purse-string wound closure. Mediates RhoA-dependent membrane blebbing. Triggers TRPC5 channel activity in a calcium-dependent signaling, by inducing its subcellular localization at the plasma membrane. Promotes cell migration (including tumor cells) and tumor metastasis. PTK2B/PYK2 activation by phosphorylation mediates ITGB2 activation and is thus essential to trigger neutrophil transmigration during acute lung injury (ALI). May regulate optic nerve head astrocyte migration. Probably involved in mitotic cytoskeletal regulation. Regulates tight junction probably by modulating ZO-1 exchange in the perijunctional actomyosin ring. Mediates burn-induced microvascular barrier injury; triggers endothelial contraction in the development of microvascular hyperpermeability by phosphorylating MLC. Essential for intestinal barrier dysfunction. Mediates Giardia spp.-mediated reduced epithelial barrier function during giardiasis intestinal infection via reorganization of cytoskeletal F-actin and tight junctional ZO-1. Necessary for hypotonicity-induced Ca2+ entry and subsequent activation of volume-sensitive organic osmolyte/anion channels (VSOAC) in cervical cancer cells. Responsible for high proliferative ability of breast cancer cells through anti-apoptosis.
Tissue specificity	Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. Telokin has been found in a wide variety of adult and fetal tissues. Accumulates in well differentiated enterocytes of the intestinal epithelium in response to tumor necrosis factor (TNF).
Posttranslational modification	Can probably be down-regulated by phosphorylation. Tyrosine phosphorylation by ABL1 increases kinase activity, reverses MLCK-mediated inhibition of Arp2/3-mediated actin polymerization, and enhances CTTN-binding. Phosphorylation by SRC at Tyr-464 and Tyr-471 promotes CTTN binding.The C-terminus is deglutamylated by AGTPBP1/CCP1, AGBL1/CCP4 and AGBL4/CCP6, leading to the formation of Myosin light chain kinase, smooth muscle, deglutamylated form. The consequences of C-terminal deglutamylation are unknown (By similarity).Acetylated at Lys-608 by NAA10/ARD1 via a calcium-dependent signaling; this acetylation represses kinase activity and reduces tumor cell migration.
Celluar localization	Cytoskeleton;Cytosol;Extracellular region or secreted;
UniPort	Q15746