Phospho-E1A-associated Protein P300 (Ser1834) Rabbit Polyclonal Antibody#abs139914
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Description
| Catalog-specification | Delivery time | USD price |
abs139914-100ug | 1-2 Weeks | 301.0 |
abs139914-50ug | 1-2 Weeks | 201.0 |
Please note that the price mentioned is for reference purposes only. If you require detailed pricing information, kindly get in touch with our sales representative Vecent. We can provide you with the necessary details so that you can make an informed decision. Thank you.
| Overview | |
catalog | abs139914 |
| Other names | p300, also known as E1A associated protein or E1A binding protein p300, is a crucial protein in cellular processes. Its primary function is as a histone acetyltransferase, responsible for modifying histone proteins and controlling gene expression. In humans, p300 is encoded by the EP300 gene. This protein, EP300, plays a vital role in various biological pathways and is involved in several cellular functions. Its importance is highlighted by the fact that mutations or disruptions in the EP300 gene can lead to Rubinstein-Taybi syndrome type 2 (RSTS2), a rare genetic disorder characterized by physical and intellectual disabilities. As an E1A binding protein, p300 interacts with the adenoviral E1A protein to regulate viral gene expression. Furthermore, p300 has been shown to interact with numerous other transcription factors, co-activators, and co-repressors. These interactions enable p300 to modulate gene transcription by acetylating histones and promoting chromatin relaxation. The acetyltransferase activity of p300 extends beyond histones. It can also acetylate non-histone proteins, such as transcription factors, DNA repair proteins, and co-regulators. By modifying these proteins, p300 influences their stability, DNA binding ability, and interaction with other proteins, thereby affecting various cellular processes. Additionally, p300 acts as a scaffold protein, bringing together multiple transcription factors and co-regulators to form transcriptional complexes. This facilitates the coordination of gene expression and the integration of multiple signaling pathways. Due to its versatility and involvement in critical cellular mechanisms, p300 has garnered significant attention in research and drug development. Modulating p300 activity or targeting specific protein-protein interactions involving p300 may hold therapeutic potential for various diseases, including cancer, neurological disorders, and cardiovascular diseases. Overall, p300, or E1A associated protein p300, plays a fundamental role in regulating gene expression and cellular processes. Its multifaceted properties make it a fascinating protein for scientific exploration and hold promise for future therapeutic interventions. |
| Source | Rabbit |
| Specificity | The antibody against E1A-associated Protein p300 (Phospho-Ser1834) is designed to specifically recognize the phosphorylated form of endogenous E1A-associated Protein p300 at Ser1834. This antibody does not detect the non-phosphorylated form of E1A-associated Protein p300. |
| Species Reactivity | Human;Mouse;Rat |
| Predictive reaction species | Xenopus;Rabbit;Pig;Sheep;Horse;Bovine;Chicken |
| Antigen | E1A-associated Protein p300 |
| Application | The recommended dilution ranges for Western Blot (WB) are 1:1000-3000, for Immunohistochemistry (IHC) are 1:50-1:200, and for ELISA (peptide) are 1:20000-1:40000. It is important to use appropriate dilutions to ensure accurate and reliable results. Variation in dilution may depend on the specific antibody and target being studied. Therefore, it is advisable to perform preliminary experiments to determine the optimal dilution for your specific experiment. Dilution factors outside the recommended ranges may result in weaker or nonspecific signals, while overly concentrated dilutions may lead to excessive background noise. |
| Immunogen | A novel peptide has been developed, which is sourced from human E1A-associated Protein p300 and is synthesized to include Phospho-Ser1834. The peptide exhibits high similarity to the original protein and has potential applications in various fields, including as a research reagent or a therapeutic agent. The development of this peptide opens up new avenues for exploring the functions and interactions of p300, as well as for investigating disease processes and developing new treatments. |
| MW | 264kDa |
| Properties | |
Concentration | 1mg/ml |
| purification | The purified rabbit serum produced an antibody that was isolated through a series of chromatography steps involving phospho- and non-phospho-peptide affinity columns. The affinity purification process allowed for the separation of the desired antibody from other components in the serum, resulting in a highly pure and specific product. |
| Clonality | Polyclonal Antibody |
| Stability & Storage | To preserve the quality of the product, it is recommended to store it at a frigid temperature of -20 °C for a duration of one year. It is advisable to avoid subjecting the product to repeated cycles of thawing and freezing as this may compromise its integrity. |
| Storage buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |
Target | |
Background | Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Mediates acetylation of histone H3 at 'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability. Mediates acetylation of histone H3 at 'Lys-27' (H3K27ac). Also functions as acetyltransferase for nonhistone targets. Acetylates 'Lys-131' of ALX1 and acts as its coactivator. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function. Acetylates HDAC1 leading to its inactivation and modulation of transcription. Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2. Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium. Promotes cardiac myocyte enlargement. Can also mediate transcriptional repression. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Acetylates FOXO1 and enhances its transcriptional activity. Acetylates BCL6 wich disrupts its ability to recruit histone deacetylases and hinders its transcriptional repressor activity. Participates in CLOCK or NPAS2-regulated rhythmic gene transcription; exhibits a circadian association with CLOCK or NPAS2, correlating with increase in PER1/2 mRNA and histone H3 acetylation on the PER1/2 promoter. Acetylates MTA1 at 'Lys-626' which is essential for its transcriptional coactivator activity (PubMed:10733570, PubMed:11430825, PubMed:11701890, PubMed:12402037, PubMed:12586840, PubMed:12929931, PubMed:14645221, PubMed:15186775, PubMed:15890677, PubMed:16617102, PubMed:16762839, PubMed:18722353, PubMed:18995842, PubMed:23415232, PubMed:23911289, PubMed:23934153, PubMed:8945521). Acetylates XBP1 isoform 2; acetylation increases protein stability of XBP1 isoform 2 and enhances its transcriptional activity (PubMed:20955178). Acetylates PCNA; acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER) (PubMed:24939902). Acetylates MEF2D. |
| Posttranslational modification | Acetylated on Lys at up to 17 positions by intermolecular autocatalysis. Deacetylated in the transcriptional repression domain (CRD1) by SIRT1, preferentially at Lys-1020. Deacetylated by SIRT2, preferentially at Lys-418, Lys-423, Lys-1542, Lys-1546, Lys-1549, Lys-1699, Lys-1704 and Lys-1707.Citrullinated at Arg-2142 by PADI4, which impairs methylation by CARM1 and promotes interaction with NCOA2/GRIP1.Methylated at Arg-580 and Arg-604 in the KIX domain by CARM1, which blocks association with CREB, inhibits CREB signaling and activates apoptotic response. Also methylated at Arg-2142 by CARM1, which impairs interaction with NCOA2/GRIP1.Sumoylated; sumoylation in the transcriptional repression domain (CRD1) mediates transcriptional repression. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3.Probable target of ubiquitination by FBXO3, leading to rapid proteasome-dependent degradation.Phosphorylated by HIPK2 in a RUNX1-dependent manner. This phosphorylation that activates EP300 happens when RUNX1 is associated with DNA and CBFB. Phosphorylated by ROCK2 and this enhances its activity. Phosphorylation at Ser-89 by AMPK reduces interaction with nuclear receptors, such as PPARG. |
| Celluar localization | Cytosol;Nucleus; |
| UniPort | Q09472 |
Western blot analysis E1A-associated Protein p300 (Phospho-Ser1834) using PMA treated HeLa whole cell lysates
This product is for research use only, not for use in diagnostic prodecures or in human.
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