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Phospho-BL-CAM (Tyr842) Rabbit Polyclonal Antibody#abs140046

Please note that the price mentioned above is for your reference only. For detailed pricing information, kindly get in touch with our sales representative, Vecent. Western blot analysis BL-CAM (Phospho-Tyr842) using PMA treated HeLa whole cell lysates This product is for research use only, not...

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Description

Catalog-specification	Delivery time	USD price
abs140046-100ug	1-2 Weeks	301.0
abs140046-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is for your reference only. For detailed pricing information, kindly get in touch with our sales representative, Vecent.

Overview
catalog	abs140046
Other names	The B cell receptor CD22, also known as B lymphocyte cell adhesion molecule (BL CAM), is a protein that plays a crucial role in the functioning of B lymphocytes. It is also referred to as CD22 antigen, CD22 molecule, CD22 protein, Lectin 2, Leu14, Lyb8, MGC130020, sialic acid binding Ig like lectin 2, Sialic acid binding immunoglobulin like lectin 2, Sialic acid-binding Ig-like lectin 2, SIGLEC 2, Siglec-2, SIGLEC2, and T cell surface antigen Leu 14. This protein is involved in the regulation of B cell activation and is critical for the development and maturation of B cells. It also plays a role in the recognition and binding of sialic acid-containing molecules, thereby mediating cell adhesion. CD22 is a potential target for therapeutic intervention in diseases such as autoimmune disorders, cancers, and infectious diseases. Understanding the structure and function of CD22 is important for developing new strategies to modulate its activity and improve human health.
Source	Rabbit
Specificity	The BL-CAM (Phospho-Tyr842) Antibody is able to identify the presence of BL-CAM that has been phosphorylated at Tyr842 at its endogenous levels. This means that when BL-CAM is in its phosphorylated state at this specific site, the antibody will be able to detect it.
Species Reactivity	Human
Antigen	BL-CAM
Application	For Western blot, the recommended dilution range is 1:1000-3000, while for immunohistochemistry, the recommended dilution range is 1:50-1:200. If you plan on using the technique of immunofluorescence or immunocytochemistry, it is recommended to dilute the antibodies at 1:100-1:500. For ELISA using peptides, the appropriate dilution range is from 1:20000-1:40000. It's important to note that all of these dilution ranges are developed based on the original text information, so using them as a guide will ensure accurate and reliable results.
Immunogen	A peptide synthesized from the human BL-CAM protein (Phospho-Tyr842) is now available. This newly developed peptide closely resembles the original sequence of BL-CAM and has been modified to contain a phosphorylated tyrosine at position 842. This modification enhances the peptide's functionality and makes it ideal for studying the signaling pathways of BL-CAM. Scientists can now utilize this highly similar peptide to investigate the role of BL-CAM in various biological processes and gain valuable insights into its functions.
MW	95kDa
Properties
Concentration	1mg/ml
purification	Purified rabbit serum was used to obtain the antibody through a purification process involving affinity purification using phospho- and non-phospho-peptide affinity columns in sequential chromatography.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality of the product, it is recommended to store it at -20 °C for one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
Tissue specificity	B-lymphocytes.
Posttranslational modification	Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.Phosphorylated on tyrosine residues by LYN.
Celluar localization	Extracellular region or secreted;Plasma Membrane;
UniPort	P20273