Phospho-Beclin-1 (Ser93/96) Rabbit Polyclonal Antibody#abs138002
Please note that the mentioned price is only for your reference. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent. Feel free to contact him for more accurate details. Western blot analysis Phospho-Beclin-1 (Ser93/96) using K562 whole cell lysates...
Description
| Catalog-specification | Delivery time | USD price |
abs138002-100ug | 1-2 Weeks | 301.0 |
abs138002-50ug | 1-2 Weeks | 201.0 |
Please note that the mentioned price is only for your reference. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent. Feel free to contact him for more accurate details.
| Overview | |
catalog | abs138002 |
| Other names | ATG6, also known as Beclin 1, is a protein involved in the process of autophagy. It is a homolog of the yeast protein VPS30 and plays a crucial role in the formation of autophagosomes. ATG6 interacts with Bcl-2 to regulate autophagy and apoptosis. Other names for the protein include Bcl-2-interacting protein beclin and Coiled-coil myosin-like BCL2-interacting protein. The human gene is referred to as BECN1 or BECN1_HUMAN, and its product, Beclin 1, is an important regulator of cellular homeostasis and survival. |
| Source | Rabbit |
| Specificity | The antibody known as Phospho-Beclin-1 (Ser93/96) has the ability to detect Beclin-1 in endogenous levels, but only when it has been phosphorylated at Ser93/96. This particular antibody is a valuable tool for researchers who are studying Beclin-1 and its role in cellular processes. By specifically detecting phosphorylated Beclin-1, this antibody can aid in the identification of potential signaling pathways and regulatory mechanisms associated with this protein. Overall, Phospho-Beclin-1 (Ser93/96) Antibody is an important tool used in many fields of research including cancer biology, autophagy, and immunology. |
| Species Reactivity | Human;Mouse;Rat |
| Antigen | Beclin-1 |
| Application | WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000 |
| Immunogen | A peptide that is artificially created and has a structure similar to human Phospho-Beclin-1 (Ser93/96) has been developed. The peptide is synthetically derived from the original protein and can be utilized in various research and medical applications. It closely mimics the structure and function of the authentic protein, allowing for more accurate and efficient investigations. This synthesized peptide offers a promising tool for further probing the intricate mechanisms of Phospho-Beclin-1 (Ser93/96) and its role in disease pathology. |
| MW | 60KD |
| Properties | |
Concentration | 1mg/ml |
| purification | The process of obtaining the antibody involved purifying rabbit serum through sequential chromatography on columns specific to phospho- and non-phospho-peptides, with the goal of achieving affinity purification. The resulting antibody can be considered highly similar to its original specimen. |
| Clonality | Polyclonal Antibody |
| Stability & Storage | One year storage is recommended at a temperature of -20 °C. It is important to avoid subjecting the store to multiple freeze/thaw cycles. |
| Storage buffer | The rabbit IgG is stored in phosphate buffered saline at a pH of 7.4, with a concentration of 150mM NaCl. It also contains 0.02% sodium azide and is mixed with 50% glycerol. It should be kept at a temperature of -20 °C for optimal storage. This product is stable for a period of 12 months starting from the date it was received. |
Target | |
Background | Plays a central role in autophagy (PubMed:23184933, PubMed:28445460). Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). Protects against infection by a neurovirulent strain of Sindbis virus (PubMed:9765397). May play a role in antiviral host defense. |
| Tissue specificity | Ubiquitous. |
| Posttranslational modification | Phosphorylation at Thr-119 by DAPK1 reduces its interaction with BCL2 and BCL2L1 and promotes induction of autophagy (PubMed:19180116). In response to autophagic stimuli, phosphorylated at serine residues by AMPK in an ATG14-dependent manner, and this phosphorylation is critical for maximally efficient autophagy (PubMed:23878393).Polyubiquitinated by NEDD4, both with 'Lys-11'- and 'Lys-63'-linkages (PubMed:21936852). 'Lys-11'-linked poyubiquitination leads to degradation and is enhanced when the stabilizing interaction partner VPS34 is depleted (PubMed:21936852). Deubiquitinated by USP10 and USP13, leading to stabilize the PIK3C3/VPS34-containing complexes (PubMed:21962518). Polyubiquitinated at Lys-402 with 'Lys-48'-linkages (PubMed:28445460). 'Lys-48'-linked poyubiquitination of Lys-402 leads to degradation (PubMed:28445460). Deubiquitinated by ATXN3, leading to stabilization (PubMed:28445460).Proteolytically processed by caspases including CASP8 and CASP3; the C-terminal fragments lack autophagy-inducing capacity and are proposed to induce apoptosis. Thus the cleavage is proposed to be an determinant to switch from autophagy to apoptosis pathways affecting cellular homeostasis including viral infections and survival of tumor cells. |
| Celluar localization | Cytosol;Endoplasmic reticulum;Endosome;Golgi apparatus;Mitochondrion;Nucleus; |
| UniPort | Q14457 |
Western blot analysis Phospho-Beclin-1 (Ser93/96) using K562 whole cell lysates
This product is for research use only, not for use in diagnostic prodecures or in human.
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