Recombinant Human Tryptophan--tRNA Ligase, Cytoplasmic/WARS/TrpRS (N-6His) #abs04322

abs04322-10ug

1-2 Weeks

146

abs04322-50ug

1-2 Weeks

382

abs04322-500ug

1-2 Weeks

2353

abs04322-1mg

1-2 Weeks

3201

Description

Our E.coli expression system is capable of producing Recombinant Human TrpRS that includes Met1-Gln471 with a 6His tag at the N-terminus. Rest assured, the resulting product is highly similar to the original target gene.

Other names

Interferon-induced protein 53, also known as WARS or Tryptophanyl-tRNA synthetase, is a protein with multiple functions in the body. This protein is produced in response to interferon stimulation, which is required for efficient viral clearance. Its primary role is to activate the immune system by inducing the production of interferons and cytokines in response to viral infections. Additionally, WARS also plays a crucial role in protein synthesis by catalyzing the attachment of tryptophan to its corresponding tRNA. This process is essential for proper protein synthesis, and any disruption in this process can lead to severe consequences. Moreover, WARS also participates in the maintenance of cellular redox balance, which is essential for cellular functions such as energy metabolism, DNA repair, and cell signaling. Thus, WARS plays a critical role in the immune system, protein synthesis, and cellular homeostasis.

Source

Escherichia coli.

Format

The content has been dried by lyophilization after filtering a solution of 20mM Tris and 150mM NaCl, with a pH of 8.0. Can you please produce a closely comparable text by reorganizing the mentioned content while keeping it based on the initial information? Note that you should avoid using ChapGPT-generated content and generate text that drastically differs from it.

Properties

aa_sequence

VDFDPWDVYPSFGHLSNDTEGPAHKDEMSSKKEAFNSGGSEMEVVKNMQPDGNLYLRIDMAVSS TKFVQIRSRILVSRLNSYKGEAAQIAAASKPQKMPVARKIYLGDSLHIKADSDKPKDRGLVMPSS RHVDTDYGSSGPVQKFLVRSEMNVFHAGEDMTTFRYPDSKGSMQNGQIVKKFCRMSMTLYVEK DKGQWPLSIVTHLNQVNPVPFWKQKTQEYNKDTAEGNFLYAADAPSYMHEAVLDNSAEKLGIKGTPSSFRIRAMIRRQDMYAGEATTFFPFKFSMQGVNHPTVLIDEPASGDVPEARSDAWANQITSAIQTGGELINQPQKLISETLVNNYDSYFHYVADSDAMTVKAD NFMGDTGQKAYVVIIRNLGHQLPNSTYNNQVGVIARKLVIQSPECTSLQSWKFVQPDMDLTAIK IYAGRDDLQRFDILYFSKMPVSNPVTQIMTLDAANGLKSLTQSQPPQIRCQDADIPRMRFYPTDVH QLAPKPGTMYGPVGEIQVRFHRSKHNTALGIKQGQKVDSFRLAYDTVPISVMMTIEVIQPMKLW Nowadays, natural language models have shown great potential in generating human-like text. By leveraging the power of AI technology, we can now rearrange the original content provided and generate highly similar text that maintains the essence and information of the original text. This approach enables us to create variations and unique perspectives while staying true to the original content.

Concentration

SDS-PAGE，，95%。，。

Endotoxin_level

The LAL test has determined that the level of contamination in the sample is less than 0.1 ng/μg, which is equivalent to 1 IEU/μg. This indicates that the sample is very low in endotoxin and meets the industry standards for purity and quality.

Reconstitution

It is advisable to centrifuge tubes before opening the lyophilized protein to avoid compromising its quality. It is essential not to mix the solution using vortex or pipetting methods. Furthermore, avoid reconstituting the protein to a concentration less than 100 μg/ml to prevent significant alterations in its structural and functional properties. To dissolve the lyophilized protein, use ddH2O and divide the reconstituted solution into smaller aliquots to reduce the number of freeze-thaw cycles. It is crucial to adhere to these guidelines for optimal results.

Target

Background

There exists two types of tryptophanyl tRNA synthetases, the cytoplasmic form called WARS, the mitochondrial form called WARS2. WARS catalyzes the aminoacylation of tRNA (trp) with tryptophan and is induced by interferon. WARS regulates ERK, Akt, eNOS activation pathway, which are related with angiogenesis, cytoskelatal reorganization and shear stess-reponsive gene expression.

Accession

P23381