Recombinant Human OSM #abs00856

abs00856-1mg

1-2 Weeks

6742

abs00856-2ug

1-2 Weeks

138

abs00856-10ug

1-2 Weeks

344

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was filtered through a 0.2 μm filter and then lyophilized to form a dry powder. To recreate this content, simply rearrange the information in a different order while maintaining the important details, such as the use of PBS and the filter size.

Properties

aa_sequence

RLPKAQDLER AAIGSCSKEY TDLMQDTSRL LDPYIRIQGL DVPKLREHCR ERPGAFPSEE TLRGLGRRGF LQTLNATLGC VLHRLADLEQ SGLNIEDLEK QLLRYMITYC RNADDSLLQL GLRRNAVLRD QREGYRDSSG VALGEYVPNN GGLLNWCCAG QALRKGVRRT RPSRKGKRLM TRGQLPR LGENYECRKL GQLNKMVQGR ELQLPFGLDG EVDGQSRHLL ATLYGELSVA EAHFAYDWSP QMAAKGYRLV IAGLFAVHKC ARCREDAIAA.
，。

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

According to the LAL method, the Recombinant HumanOSM has been determined to have a concentration of less than 1 EU/μg. It is important to note that this information is based on the original text provided.

Activity

The standard and the ED exhibit comparable biological activity, showcasing their full potential. Please create text that closely resembles the original content but reorganized to adhere to the given instructions.₅₀The specific activity of our product can be determined by conducting a cell proliferation assay using human TF-1 cells. Our research has shown that the concentration of the product where cellular proliferation is observed is less than 2 ng/ml, which corresponds to a specific activity of over 5.0 × 10. We maintain strict standards to ensure the accuracy of our findings, and we are confident in the quality and effectiveness of our product.⁵ IU/mg.

Reconstitution

To ensure the contents are properly settled, it is advised to briefly centrifuge the vial before opening. Reconstitute the contents in sterile distilled water or an aqueous buffer containing 0.1% BSA, aiming for a concentration of 0.1-1.0 mg/mL. It is recommended to divide the stock solutions into smaller working aliquots and store them at temperatures of ≤ -20 °C. Any further dilutions should be prepared using appropriate buffered solutions.

Stability & Storage

For optimal storage conditions of this lyophilized preparation, it is recommended to keep it at -20 °C while desiccated. However, it can also be stored at 2-8 °C. Once reconstituted, the preparation is stable for up to one week at 2-8 °C. To ensure maximal stability, it is advised to divide the reconstituted preparation into working aliquots and store them at a temperature between -20 °C to -70 °C. It is important to avoid multiple freeze/thaw cycles as these can decrease the stability of the preparation.

Target

Accession

P13725

Gene IDs

5008

References

References

1. Tanaka M, Miyajima A. 2003. Rev Physiol Biochem Pharmacol. 149:39-52.
2. Mosley B, De Imus C, Friend D, et al. 1996. J Biol Chem. 271:32635-43.
3. Malik N, Kallestad JC, Gunderson NL, et al. 1989. Mol Cell Biol. 9:2847-53.
4. Brown TJ, Rowe JM, Liu JW, et al. 1991. J Immunol. 147:2175-80.